ID   HEK293T GALE KO clone 4
AC   CVCL_VR11
DR   Wikidata; Q94095755
RX   PubMed=28654657;
CC   Characteristics: Devoid of all O-glycosylation. But with the addition of galactose and GalNAc to the culture media, salvage pathways are able to compensate for the lack of GALE enzymatic activity and restore O-linked glycosylation.
CC   Knockout cell: Method=CRISPR/Cas9; HGNC; 4116; GALE.
CC   Transfected with: UniProtKB; P00552; Transposon Tn5 neo.
CC   Transformant: NCBI_TaxID; 28285; Adenovirus 5.
CC   Transformant: NCBI_TaxID; 1891767; Simian virus 40 (SV40) [Note=tsA].
CC   Derived from site: In situ; Fetal kidney; UBERON=UBERON_0002113.
OX   NCBI_TaxID=9606; ! Homo sapiens (Human)
HI   CVCL_0063 ! HEK293T
SX   Female
AG   Fetus
CA   Transformed cell line
DT   Created: 13-11-18; Last updated: 05-10-23; Version: 7
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RX   PubMed=28654657; DOI=10.1371/journal.pone.0179949;
RA   Termini J.M., Silver Z.A., Connor B., Antonopoulos A., Haslam S.M.,
RA   Dell A., Desrosiers R.C.;
RT   "HEK293T cell lines defective for O-linked glycosylation.";
RL   PLoS ONE 12:E0179949-E0179949(2017).
//