ID   HUES8 iCas9
AC   CVCL_VR10
DR   Wikidata; Q94308392
RX   PubMed=24931489;
CC   From: Memorial Sloan Kettering Cancer Center; New York; USA.
CC   Characteristics: Cell line with robust Dox-inducible expression of Cas9. Can be used for rapid and highly efficient generation of biallelic knockout hPSCs for loss-of-function studies.
CC   Characteristics: Using TALEN two constructs SA-2A-Puro-Cas9-TRE and SA-2A-Neo-M2rtTA were introduced in the AAVS1 safe harbor locus, one in each allele.
CC   Transfected with: UniProtKB; Q99ZW2; Streptomyces pyogenes Cas9.
CC   Transfected with: UniProtKB; P00552; Transposon Tn5 neo.
CC   Transfected with: UniProtKB; P13249; S.alboniger pac (PuroR).
CC   Derived from site: In situ; Blastocyst; UBERON=UBERON_0000358.
CC   Cell type: Embryonic stem cell; CL=CL_0002322.
ST   Source(s): MSKCC
ST   Amelogenin: X,Y
ST   CSF1PO: 10,12
ST   D10S1248: 14
ST   D12S391: 16,18
ST   D13S317: 12,14
ST   D16S539: 9,12
ST   D18S51: 12,16
ST   D19S433: 13,15
ST   D1S1656: 15
ST   D21S11: 28,29
ST   D22S1045: 14,15,16
ST   D2S1338: 17
ST   D2S441: 11,15
ST   D3S1358: 15,19
ST   D5S818: 11
ST   D7S820: 11,12
ST   D8S1179: 13
ST   DYS391: 10
ST   FGA: 20,25
ST   Penta D: 10,11
ST   Penta E: 7,10
ST   TH01: 7,9
ST   TPOX: 8,11
ST   vWA: 15,19
OX   NCBI_TaxID=9606; ! Homo sapiens (Human)
HI   CVCL_B207 ! HUES 8
SX   Male
AG   Blastocyst stage
CA   Embryonic stem cell
DT   Created: 13-11-18; Last updated: 02-05-24; Version: 7
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RX   PubMed=24931489; DOI=10.1016/j.stem.2014.05.018;
RA   Gonzalez F., Zhu Z.-R., Shi Z.-D., Lelli K., Verma N., Li Q.V.,
RA   Huangfu D.-W.;
RT   "An iCRISPR platform for rapid, multiplexable, and inducible genome
RT   editing in human pluripotent stem cells.";
RL   Cell Stem Cell 15:215-226(2014).
//