ID   AR-EcoScreen GR KO M1
AC   CVCL_VN86
SY   AR-EcoScreen GR KnockOut Mutant 1
DR   JCRB; JCRB1761
DR   Wikidata; Q93325715
RX   PubMed=28803809;
CC   Characteristics: Used for rapid evaluation of in vitro androgen receptor transcriptional activation. The NR3C1/GR knockout renders the AR-EcoScreen assay insensitive to glucocorticoid activation.
CC   Characteristics: The luciferase reporter gene is under the control of a construct bearing four tandem repeats of a prostate C3 gene androgen-responsive element driven by a minimal heat shock protein promoter.
CC   Knockout cell: Method=CRISPR/Cas9; HGNC; 7978; NR3C1.
CC   Transfected with: HGNC; 644; AR.
CC   Transfected with: UniProtKB; P27652; Renilla-type luciferase.
CC   Derived from site: In situ; Ovary; UBERON=UBERON_0000992.
CC   Cell type: Epithelial cell of ovary; CL=CL_2000064.
OX   NCBI_TaxID=10029; ! Cricetulus griseus (Chinese hamster)
HI   CVCL_8544 ! AR-EcoScreen
SX   Female
AG   Adult
CA   Spontaneously immortalized cell line
DT   Created: 07-09-18; Last updated: 30-01-24; Version: 6
//
RX   PubMed=28803809; DOI=10.1016/j.tiv.2017.08.004;
RA   Zwart N., Andringa D., de Leeuw W.-J., Kojima H., Iida M.,
RA   Houtman C.J., de Boer J., Kool J., Lamoree M.H., Hamers T.;
RT   "Improved androgen specificity of AR-EcoScreen by CRISPR based
RT   glucocorticoid receptor knockout.";
RL   Toxicol. In Vitro 45:1-9(2017).
//