ID   hTERT NF1 ipNF05.5
AC   CVCL_UI71
SY   ipNF05.5
DR   ABM; T0180
DR   ATCC; CRL-3388
DR   Wikidata; Q94305827
RX   PubMed=27617404;
RX   PubMed=29893754;
WW   Info; NTAP; -; https://www.n-tap.org/for-researchers/cell-model-systems
CC   Population: Caucasian.
CC   Doubling time: ~59 hours (ATCC=CRL-3388).
CC   Karyotypic information: 46,XY,t(2;3)(q23;p26) [18]; 47,sl,+9 [2] (ATCC=CRL-3388).
CC   Sequence variation: Gene deletion; HGNC; HGNC:7765; NF1; Zygosity=Heterozygous; Note=Somatic LOH (PubMed=27617404).
CC   Sequence variation: Mutation; HGNC; HGNC:7765; NF1; Simple; p.Leu1153Thrfs*42 (c.3456dupA) (3456_3457insA); ClinVar=VCV000656975; Zygosity=Heterozygous; Note=Germline (PubMed=27617404).
CC   Genetic integration: Method=Transduction; Gene=HGNC; HGNC:11730; TERT.
CC   Genetic integration: Method=Transduction; Gene=MGI; MGI:88357; Cdk4.
CC   Genetic integration: Method=Transduction; Gene=UniProtKB; P13249; S.alboniger pac (PuroR).
CC   Genetic integration: Method=Transduction; Gene=UniProtKB; P00552; Transposon Tn5 neo.
CC   Omics: Genomics; Whole exome sequencing.
CC   Omics: Transcriptomics; RNAseq.
CC   Omics: Variations; SNP array analysis.
CC   Caution: This cell line was also termed 'ipNF05.5 (single clone)', but this is a misnomer as this cell line is not clonal (personal communication of Allaway, Robert J.).
CC   Derived from site: In situ; Peripheral nervous system, nerve; UBERON=UBERON_0001021.
CC   Cell type: Schwann cell; CL=CL_0002573.
ST   Source(s): ATCC=CRL-3388; PubMed=27617404
ST   Amelogenin: X,Y
ST   CSF1PO: 10,11
ST   D13S317: 8,12
ST   D16S539: 11,12
ST   D18S51: 15,19
ST   D19S433: 14,16
ST   D21S11: 30
ST   D2S1338: 17
ST   D3S1358: 14,17
ST   D5S818: 12
ST   D7S820: 8,12
ST   D8S1179: 13,14
ST   FGA: 21
ST   Penta D: 9,13
ST   Penta E: 5,10
ST   TH01: 6,9.3
ST   TPOX: 8,12
ST   vWA: 17,18
DI   NCIt; C3273; Neurofibromatosis type 1
DI   ORDO; Orphanet_636; Neurofibromatosis type 1
OX   NCBI_TaxID=9606; ! Homo sapiens (Human)
OI   CVCL_UI72 ! hTERT NF1 ipNF05.5 (Mixed clones)
OI   CVCL_UI73 ! hTERT NF1 ipnNF09.4
SX   Male
AG   22Y
CA   Telomerase immortalized cell line
DT   Created: 25-02-19; Last updated: 10-04-25; Version: 16
//
RX   PubMed=27617404; DOI=10.1038/labinvest.2016.88;
RA   Li H., Chang L.-J., Neubauer D.R., Muir D.F. 4th, Wallace M.R.;
RT   "Immortalization of human normal and NF1 neurofibroma Schwann cells.";
RL   Lab. Invest. 96:1105-1115(2016).
//
RX   PubMed=29893754; DOI=10.1038/sdata.2018.106; PMCID=PMC5996849;
RA   Ferrer M., Gosline S.J.C., Stathis M., Zhang X.-H., Guo X.-D., Guha R.,
RA   Ryman D.A., Wallace M.R., Kasch-Semenza L., Hao H.-P., Ingersoll R.G.,
RA   Mohr D., Thomas C.J., Verma S., Guinney J.H., Blakeley J.O.;
RT   "Pharmacological and genomic profiling of neurofibromatosis type 1
RT   plexiform neurofibroma-derived Schwann cells.";
RL   Sci. Data 5:180106.1-180106.12(2018).
//