ID   A-375 MEK1 p.Q56P
AC   CVCL_UD28
SY   A-375 MEK1Q56P; MEK1 Mutant-A375 Isogenic Cell Line
DR   ATCC; CRL-1619IG-3
DR   cancercelllines; CVCL_UD28
DR   Wikidata; Q93311031
CC   Population: Caucasian.
CC   Characteristics: Exhibits significant resistance to the BRAF inhibitors Dabrafineb and Vemurafenib, as well as the MAP2K1 inhibitor trametinib when compared to its parental cell line. Displays increased sensitivity to combination MAP2K1/BRAF inhibitor treatments, making this line an ideal model system for the development of novel combination therapies targeting multiple points in the RAS-RAF-MEK-ERK-MAPK signaling pathway, as well as for the screening of new potential BRAF and MAP2K1 inhibitors (ATCC=CRL-1619IG-3).
CC   Doubling time: ~19 hours (ATCC=CRL-1619IG-3).
CC   Sequence variation: Mutation; HGNC; 1097; BRAF; Simple; p.Val600Glu (c.1799T>A); ClinVar=VCV000013961; Zygosity=Homozygous (from parent cell line).
CC   Sequence variation: Mutation; HGNC; 1787; CDKN2A; Simple; p.Glu61Ter (c.181G>T) (p.Gly75Val, c.224G>T); ClinVar=VCV000376309; Zygosity=Homozygous (from parent cell line).
CC   Sequence variation: Mutation; HGNC; 1787; CDKN2A; Simple; p.Glu69Ter (c.205G>T) (p.Gly83Val, c.248G>T); ClinVar=VCV000376308; Zygosity=Homozygous (from parent cell line).
CC   Sequence variation: Mutation; HGNC; 6840; MAP2K1; Simple_edited; p.Gln56Pro (c.167A>C); ClinVar=VCV000375978; Zygosity=Heterozygous; Note=By CRISPR/Cas9 (ATCC=CRL-1619IG-3).
CC   Sequence variation: Mutation; HGNC; 11730; TERT; Simple; c.1-146C>T (c.250C>T) (C250T); Zygosity=Unspecified; Note=In promoter (from parent cell line).
CC   Derived from site: In situ; Skin; UBERON=UBERON_0002097.
ST   Source(s): ATCC=CRL-1619IG-3
ST   Amelogenin: X
ST   CSF1PO: 11,12
ST   D13S317: 11,14
ST   D16S539: 9
ST   D5S818: 12
ST   D7S820: 9
ST   TH01: 8
ST   TPOX: 8,10
ST   vWA: 16,17
DI   NCIt; C3802; Amelanotic melanoma
OX   NCBI_TaxID=9606; ! Homo sapiens (Human)
HI   CVCL_0132 ! A-375
SX   Female
AG   54Y
CA   Cancer cell line
DT   Created: 13-11-18; Last updated: 02-05-24; Version: 11
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