ID   tsBN2
AC   CVCL_U467
DR   CLO; CLO_0051408
DR   RCB; RCB1264
DR   Wikidata; Q54973104
RX   PubMed=694723;
RX   PubMed=1851087;
RX   PubMed=2300055;
RX   PubMed=3785187;
CC   Characteristics: At the nonpermissive temperature (39.5 Celsius), the chromatin of tsBN2 cells is prematurely condensed, and the cells die (PubMed=694723).
CC   Sequence variation: Mutation; UniProtKB; P23800; Rcc1; Simple; p.Ser256Phe (c.767C>T); Zygosity=Homozygous (PubMed=2300055).
CC   Transformant: ChEBI; CHEBI_21759; N-methyl-N'-nitro-N-nitrosoguanidine (MNNG).
CC   Derived from site: In situ; Kidney; UBERON=UBERON_0002113.
OX   NCBI_TaxID=10036; ! Mesocricetus auratus (Golden hamster)
HI   CVCL_1915 ! BHK-21 clone 13
SX   Male
AG   1D
CA   Spontaneously immortalized cell line
DT   Created: 03-02-14; Last updated: 19-12-24; Version: 9
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RX   PubMed=694723; DOI=10.1007/BF01542846;
RA   Nishimoto T., Basilico C.;
RT   "Analysis of a method for selecting temperature-sensitive mutants of
RT   BHK cells.";
RL   Somatic Cell Genet. 4:323-340(1978).
//
RX   PubMed=1851087; DOI=10.1002/j.1460-2075.1991.tb07675.x; PMCID=PMC452820;
RA   Nishitani H., Ohtsubo M., Yamashita K., Iida H., Pines J., Yasudo H.,
RA   Shibata Y., Hunter T., Nishimoto T.;
RT   "Loss of RCC1, a nuclear DNA-binding protein, uncouples the completion
RT   of DNA replication from the activation of cdc2 protein kinase and
RT   mitosis.";
RL   EMBO J. 10:1555-1564(1991).
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RX   PubMed=2300055; DOI=10.1128/mcb.10.2.577-584.1990; PMCID=PMC360843;
RA   Uchida S., Sekiguchi T., Nishitani H., Miyauchi K., Ohtsubo M.,
RA   Nishimoto T.;
RT   "Premature chromosome condensation is induced by a point mutation in
RT   the hamster RCC1 gene.";
RL   Mol. Cell. Biol. 10:577-584(1990).
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RX   PubMed=3785187; DOI=10.1128/mcb.6.6.2027-2032.1986; PMCID=PMC367742;
RA   Kai R., Ohtsubo M., Sekiguchi M., Nishimoto T.;
RT   "Molecular cloning of a human gene that regulates chromosome
RT   condensation and is essential for cell proliferation.";
RL   Mol. Cell. Biol. 6:2027-2032(1986).
//