ID   MAC-T
AC   CVCL_U226
SY   Mac-T; MacT; MAC-T3; Mammary Alveolar Cells-large T antigen
DR   BCRJ; 0315
DR   GEO; GSE46476
DR   Wikidata; Q54903957
RX   CelloPub=CLPUB00665;
RX   DOI=10.4172/2329-888X.1000103;
RX   Patent=US5227301;
RX   Patent=US5455164;
RX   PubMed=1423654;
RX   PubMed=1659986;
RX   PubMed=7540186;
RX   PubMed=14550508;
RX   PubMed=26659725;
RX   PubMed=30055215;
CC   Group: Patented cell line.
CC   Registration: International Depositary Authority, American Type Culture Collection (ATCC); CRL-10274.
CC   Doubling time: ~17 hours (PubMed=1659986); 48 hours (Note=In serum-free medium) (PubMed=7540186).
CC   Transfected with: UniProtKB; P00552; Transposon Tn5 neo.
CC   Transformant: NCBI_TaxID; 1891767; Simian virus 40 (SV40) [Note=tsA58].
CC   Omics: Transcriptome analysis by microarray.
CC   Anecdotal: Nexia, a former Canadian biotech company was orignally developed to make lactose-free free milk using MAC-T.
CC   Derived from site: In situ; Mammary gland; UBERON=UBERON_0001911.
CC   Cell type: Mammary alveolar epithelial cell; CL=CL_0002325.
CC   Breed/subspecies: Holstein.
OX   NCBI_TaxID=9913; ! Bos taurus (Bovine)
HI   CVCL_B3UP ! LMH17
SX   Female
AG   Adult
CA   Transformed cell line
DT   Created: 03-02-14; Last updated: 05-10-23; Version: 16
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RX   CelloPub=CLPUB00665;
RA   Huynh H.T.;
RT   "Establishment of bovine mammary epithelial cell lines: an in vitro
RT   model for lactation.";
RL   Thesis MSc (1990), McGill University Montreal, Canada.
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RX   DOI=10.4172/2329-888X.1000103;
RA   Hosseini A., Sharma R., Bionaz M., Loor J.J.;
RT   "Transcriptomics comparisons of Mac-T cells versus mammary tissue
RT   during late pregnancy and peak lactation.";
RL   Adv. Dairy Res. 1:103-103(2013).
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RX   Patent=US5227301;
RA   Turner J.D., Huynh H.T.;
RT   "Immortalized bovine mammary epithelial cell line.";
RL   Patent number US5227301, 13-Jul-1993.
//
RX   Patent=US5455164;
RA   Turner J.D.;
RT   "Ruminant immortalized mammary epithelial cell lines.";
RL   Patent number US5455164, 03-Oct-1995.
//
RX   PubMed=1423654; DOI=10.1016/S0309-1651(06)80052-4;
RA   Rejman J.J., Oliver S.P., Muenchen R.A., Turner J.D.;
RT   "Proliferation of the MAC-T bovine mammary epithelial cell line in the
RT   presence of mammary secretion whey proteins.";
RL   Cell Biol. Int. Rep. 16:993-1001(1992).
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RX   PubMed=1659986; DOI=10.1016/0014-4827(91)90422-Q;
RA   Huynh H.T., Robitaille G., Turner J.D.;
RT   "Establishment of bovine mammary epithelial cells (MAC-T): an in vitro
RT   model for bovine lactation.";
RL   Exp. Cell Res. 197:191-199(1991).
//
RX   PubMed=7540186; DOI=10.3168/jds.S0022-0302(95)76662-0;
RA   Zavizion B., Gorewit R.C., Politis I.;
RT   "Subcloning the MAC-T bovine mammary epithelial cell line: morphology,
RT   growth properties, and cytogenetic analysis of clonal cells.";
RL   J. Dairy Sci. 78:515-527(1995).
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RX   PubMed=14550508; DOI=10.1016/S0739-7240(03)00062-6;
RA   Berry S.D.K., Weber Nielsen M.S., Sejrsen K., Pearson R.E.,
RA   Boyle P.L., Akers R.M.;
RT   "Use of an immortalized bovine mammary epithelial cell line (MAC-T) to
RT   measure the mitogenic activity of extracts from heifer mammary tissue:
RT   effects of nutrition and ovariectomy.";
RL   Domest. Anim. Endocrinol. 25:245-253(2003).
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RX   PubMed=26659725; DOI=10.1152/ajpcell.00261.2015;
RA   Arevalo Turrubiarte M., Perruchot M.-H., Finot L., Mayeur F.,
RA   Dessauge F.;
RT   "Phenotypic and functional characterization of two bovine mammary
RT   epithelial cell lines in 2D and 3D models.";
RL   Am. J. Physiol. Cell Physiol. 310:C348-C356(2016).
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RX   PubMed=30055215; DOI=10.1016/j.virusres.2018.07.013;
RA   Martinez Cuesta L., Nieto Farias M.V., Lendez P.A., Barone L.,
RA   Perez S.E., Dolcini G.L., Ceriani M.C.;
RT   "Stable infection of a bovine mammary epithelial cell line (MAC-T)
RT   with bovine leukemia virus (BLV).";
RL   Virus Res. 256:11-16(2018).
//