ID   NKNT-3
AC   CVCL_S905
DR   Wikidata; Q54930683
RX   PubMed=10678831;
RX   PubMed=10937342;
RX   PubMed=16970284;
CC   Group: Serum/protein free medium cell line.
CC   Characteristics: The SV40 T antigen is flanked by LoxP recombination targets so that it can be excised by Cre/Lox site-specific recombination.
CC   Transfected with: UniProtKB; P00557; Escherichia coli hygromycin-B 4-O-kinase (hph) (HygR).
CC   Transfected with: UniProtKB; Q9QNF7; Human herpesvirus 1 TK.
CC   Transformant: NCBI_TaxID; 1891767; Simian virus 40 (SV40).
CC   Derived from site: In situ; Liver; UBERON=UBERON_0002107.
CC   Cell type: Hepatocyte; CL=CL_0000182.
OX   NCBI_TaxID=9606; ! Homo sapiens (Human)
SX   Sex unspecified
AG   Adult
CA   Transformed cell line
DT   Created: 03-02-14; Last updated: 05-10-23; Version: 13
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RX   PubMed=10678831; DOI=10.1126/science.287.5456.1258;
RA   Kobayashi N., Fujiwara T., Westerman K.A., Inoue Y., Sakaguchi M.,
RA   Noguchi H., Miyazaki M., Cai J., Tanaka N., Fox I.J., Leboulch P.;
RT   "Prevention of acute liver failure in rats with reversibly
RT   immortalized human hepatocytes.";
RL   Science 287:1258-1262(2000).
//
RX   PubMed=10937342;
RA   Kobayashi N., Noguchi H., Watanabe T., Matsumura T., Totsugawa T.,
RA   Fujiwara T., Westerman K.A., Leboulch P., Fox I.J., Tanaka N.;
RT   "Establishment of a tightly regulated human cell line for the
RT   development of hepatocyte transplantation.";
RL   Hum. Cell 13:7-13(2000).
//
RX   PubMed=16970284; DOI=10.3727/000000006783981873;
RA   Hoekstra R., Deurholt T., ten Bloemendaal L., Desille M.,
RA   van Wijk A.C.W.A., Clement B., Oude Elferink R.P.J., van Gulik T.M.,
RA   Chamuleau R.A.F.M.;
RT   "Assessment of in vitro applicability of reversibly immortalized NKNT-3
RT   cells and clonal derivatives.";
RL   Cell Transplant. 15:423-433(2006).
//