ID   K-562 CRISPRa+SaCas9 clone C7
AC   CVCL_RY27
DR   Wikidata; Q54899216
RX   PubMed=29334369;
CC   Population: Caucasian.
CC   Characteristics: This cell line is transfected by a nuclease-deficient mutant of SpCas9 (dCas9) under the control of a promoter containing 4 copies of the activating domain of HSV-1 VP16 (termed VP64) and to 24 copies of a short peptide epitope (from yeast GCN4) which is termed SunTag.
CC   Sequence variation: Gene fusion; HGNC; 76; ABL1 + HGNC; 1014; BCR; Name(s)=BCR-ABL1, BCR-ABL; Note=BCR exon 14 fused to ABL1 exon 2 (b3a2 transcript) (from parent cell line).
CC   Sequence variation: Mutation; HGNC; 11998; TP53; Simple; p.Gln136fs*13 (c.406_407insC); Zygosity=Homozygous (from parent cell line).
CC   Transfected with: UniProtKB; J7RUA5; Staphylococcus aureus Cas9.
CC   Transfected with: UniProtKB; Q99ZW2; Streptomyces pyogenes Cas9 (with p.Asp10Ala and p.His840Ala = dCas9).
CC   Derived from site: In situ; Pleural effusion; UBERON=UBERON_0000175.
DI   NCIt; C9110; Blast phase chronic myelogenous leukemia, BCR-ABL1 positive
DI   ORDO; Orphanet_521; Chronic myeloid leukemia
OX   NCBI_TaxID=9606; ! Homo sapiens (Human)
HI   CVCL_RY26 ! K-562 CRISPRa
SX   Female
AG   53Y
CA   Cancer cell line
DT   Created: 14-05-18; Last updated: 02-05-24; Version: 12
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RX   PubMed=29334369; DOI=10.1038/nbt.4062;
RA   Boettcher M., Tian R.-L., Blau J.A., Markegard E., Wagner R.T., Wu D.,
RA   Mo X.-L., Biton A., Zaitlen N., Fu H.-A., McCormick F., Kampmann M.,
RA   McManus M.T.;
RT   "Dual gene activation and knockout screen reveals directional
RT   dependencies in genetic networks.";
RL   Nat. Biotechnol. 36:170-178(2018).
//