ID   HA-R-Spondin1-Fc 293T
AC   CVCL_RU08
SY   HA-R-Spondin-Fc293T
DR   Wikidata; Q54872280
RX   PubMed=19398967;
WW   Provider; AMSBIO; -; https://www.amsbio.com/r-spondin-rspo1-cells/
CC   Characteristics: Expresses mouse Fc-Rspo1 which consist of the fusion of mouse IgG2a Fc region to the N-terminal of Rspo1 with a N-terminal HA epitope tag (AMSBIO).
CC   Genetic integration: Method=Transfection; Gene=MGI; MGI:2183426; Rspo1 (Note=With a N-terminal HA tag).
CC   Genetic integration: Method=Transfection; Gene=UniProtKB; P17493; Streptoalloteichus hindustanus ble (BleoR).
CC   Genetic integration: Method=Transfection; Gene=UniProtKB; P03070; SV40 large T antigen.
CC   Genetic integration: Method=Transfection; Gene=UniProtKB; P00552; Transposon Tn5 neo.
CC   Transformant: NCBI_TaxID; 28285; Adenovirus 5.
CC   Transformant: NCBI_TaxID; 1891767; Simian virus 40 (SV40) (Note=tsA).
CC   Caution: Could be identical to 293T R-Spondin1 expressing (Cellosaurus=CVCL_RU09) distributed by Millipore. Both cell line product pages cite PubMed=19398967 but the Millipore page states "Cell line is not the published cell line from the Kuo lab, but rather a related cell line developed in the same lab using the reference protocol".
CC   Derived from site: In situ; Fetal kidney; UBERON=UBERON_0002113.
OX   NCBI_TaxID=9606; ! Homo sapiens (Human)
HI   CVCL_0063 ! HEK293T
SX   Female
AG   Fetus
CA   Transformed cell line
DT   Created: 05-03-18; Last updated: 10-04-25; Version: 10
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RX   PubMed=19398967; DOI=10.1038/nm.1951; PMCID=PMC2919216;
RA   Ootani A., Li X.-N., Sangiorgi E., Ho Q.T., Ueno H., Toda S.,
RA   Sugihara H., Fujimoto K., Weissman I.L., Capecchi M.R., Kuo C.J.;
RT   "Sustained in vitro intestinal epithelial culture within a
RT   Wnt-dependent stem cell niche.";
RL   Nat. Med. 15:701-706(2009).
//