ID   U2OS His10-SUMO-2-K0-Q87R
AC   CVCL_RJ63
DR   PRIDE; PXD004927
DR   PRIDE; PXD005296
DR   Wikidata; Q54973666
RX   PubMed=25755297;
RX   PubMed=28112733;
CC   Population: Caucasian.
CC   Characteristics: Transfected with a decahistidine (His10) tagged lysine-deficient (K0) and Q87R SUMO2 and GFP separated by an internal ribosome entry site (IRES).
CC   Sequence variation: Mutation; HGNC; HGNC:9277; PPM1D; Simple; p.Arg458Ter (c.1372C>T); ClinVar=VCV002445214; Zygosity=Heterozygous (from parent cell line).
CC   Genetic integration: Method=Transfection/transduction; Gene=FPbase; R9NL8; eGFP (Note=Enhanced GFP).
CC   Genetic integration: Method=Transfection/transduction; Gene=HGNC; HGNC:11125; SUMO2 (Note=With p.Lys11Arg, p.Lys32Arg, p.Lys32Arg, p.Lys34Arg, p.Lys41Arg, p.Lys44Arg and p.Gln87Arg).
CC   Omics: Proteomics; PTM; Sumoylation.
CC   Derived from site: In situ; Bone, tibia; UBERON=UBERON_0000979.
DI   NCIt; C9145; Osteosarcoma
DI   ORDO; Orphanet_668; Osteosarcoma
OX   NCBI_TaxID=9606; ! Homo sapiens (Human)
HI   CVCL_0042 ! U2OS
SX   Female
AG   15Y
CA   Cancer cell line
DT   Created: 05-03-18; Last updated: 10-04-25; Version: 10
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RX   PubMed=25755297; DOI=10.1074/mcp.O114.044792; PMCID=PMC4424410;
RA   Xiao Z.-Y., Chang J.-G., Hendriks I.A., Sigurosson J.O., Olsen J.V.,
RA   Vertegaal A.C.O.;
RT   "System-wide analysis of sumoylation dynamics in response to
RT   replication stress reveals novel small ubiquitin-like modified target
RT   proteins and acceptor lysines relevant for genome stability.";
RL   Mol. Cell. Proteomics 14:1419-1434(2015).
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RX   PubMed=28112733; DOI=10.1038/nsmb.3366;
RA   Hendriks I.A., Lyon D., Young C., Jensen L.J., Vertegaal A.C.O.,
RA   Nielsen M.L.;
RT   "Site-specific mapping of the human SUMO proteome reveals
RT   co-modification with phosphorylation.";
RL   Nat. Struct. Mol. Biol. 24:325-336(2017).
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