ID   BIONi010-C-13
SY   BIONi010-C + NGN2 #I7-26
DR   BioSamples; SAMEA103988285
DR   EBiSC; BIONi010-C-13
DR   ECACC; 66540561
DR   hPSCreg; BIONi010-C-13
DR   SKIP; SKIP002903
DR   Wikidata; Q54796758
RX   PubMed=33610017;
CC   From: Bioneer A/S; Horsholm; Denmark.
CC   Population: African.
CC   Characteristics: Expression of NEUROG2/NGN2 is doxycycline-inducible. Using TALEN NEUROG2 under the control of a tetracycline response element and a 2A-Puro was inserted into one allele of AAVS1 safe harbor locus. At the second locus a 2A-Neo-CAG-rtTA construct was inserted (PubMed=33610017).
CC   Transfected with: HGNC; 13805; NEUROG2.
CC   Transfected with: UniProtKB; P04483; Escherichia coli Tn10 tetR.
CC   Transfected with: UniProtKB; P00552; Transposon Tn5 neo.
CC   Transfected with: UniProtKB; P13249; S.alboniger pac (PuroR).
CC   Transfected with: UniProtKB; P06492; Human herpesvirus 1 VP16 (UL28) (with p.Met1_Arg360del).
CC   Discontinued: ECACC; 66540561; true.
CC   Derived from site: In situ; Skin, dermis; UBERON=UBERON_0002067.
CC   Cell type: Fibroblast of skin; CL=CL_0002620.
OX   NCBI_TaxID=9606; ! Homo sapiens (Human)
HI   CVCL_1E68 ! BIONi010-C
SX   Male
AG   18Y
CA   Induced pluripotent stem cell
DT   Created: 05-03-18; Last updated: 02-05-24; Version: 12
RX   PubMed=33610017; DOI=10.1016/j.scr.2021.102240;
RA   Schmid B., Holst B., Poulsen U.B., Jorring I., Clausen C., Rasmussen M.,
RA   Mau-Holzmann U.A., Steeg R., Nuthall H., Ebneth A.,
RA   Cabrera-Socorro A.;
RT   "Generation of two gene edited iPSC-lines carrying a DOX-inducible
RT   NGN2 expression cassette with and without GFP in the AAVS1 locus.";
RL   Stem Cell Res. 52:102240-102240(2021).