ID   HT1080-hNIS-Neo/Fluc-Puro
AC   CVCL_RA14
DR   cancercelllines; CVCL_RA14
DR   Imanis; CL105
DR   Wikidata; Q54896562
CC   Population: Caucasian.
CC   Characteristics: Reporter cell line, transfected with both the LV-hNIS-IRES-Neo and LV-Fluc-P2A-Puro vectors. LV-hNIS-IRES-Neo encodes for human SLC5A5 linked via an internal ribosomal entry site (IRES) to the Neo gene and under control of the spleen focus-forming virus (SFFV) promoter. LV-Fluc-P2A-Puro encodes for luciferase linked via a P2A cleavage peptide to the pac gene and under control of the spleen focus-forming virus (SFFV) promoter (Imanis=CL105).
CC   Sequence variation: Gene deletion; HGNC; 1787; CDKN2A; Zygosity=Homozygous (from parent cell line).
CC   Sequence variation: Mutation; HGNC; 5382; IDH1; Simple; p.Arg132Cys (c.394C>T); ClinVar=VCV000375891; Zygosity=Heterozygous (from parent cell line).
CC   Sequence variation: Mutation; HGNC; 7989; NRAS; Simple; p.Gln61Lys (c.181C>A); ClinVar=VCV000073058; Zygosity=Heterozygous (from parent cell line).
CC   Transfected with: HGNC; 11040; SLC5A5.
CC   Transfected with: UniProtKB; P08659; Firefly luciferase.
CC   Transfected with: UniProtKB; P00552; Transposon Tn5 neo.
CC   Transfected with: UniProtKB; P13249; S.alboniger pac (PuroR).
CC   Derived from site: In situ; Bone, pelvis, acetabulum; UBERON=UBERON_0001269.
DI   NCIt; C3043; Fibrosarcoma
DI   ORDO; Orphanet_2030; Fibrosarcoma
OX   NCBI_TaxID=9606; ! Homo sapiens (Human)
HI   CVCL_0317 ! HT-1080
SX   Male
AG   35Y
CA   Cancer cell line
DT   Created: 05-03-18; Last updated: 02-05-24; Version: 14
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