ID   CT26.WT-mNIS-Neo/eGFP-Puro
AC   CVCL_RA06
DR   Imanis; CL045
DR   Wikidata; Q54814718
CC   Characteristics: Reporter cell line, transduced with both the LV-mNIS-P2A-Neo and LV-eGFP-PGKp-Puro vectors. LV-mNIS-P2A-Neo encodes for mouse Slc5a5 linked via a P2A cleavage peptide to the neo gene under control of the spleen focus-forming virus (SFFV) promoter. LV-eGFP-PGKp-Puro encodes for eGFP under control of the spleen focus-forming virus (SFFV) promoter and the PuroR gene under the control of the mouse PGK promoter (Imanis=CL045).
CC   Sequence variation: Gene deletion; MGI; MGI:104738; Cdkn2a; Zygosity=Homozygous (from parent cell line).
CC   Sequence variation: Mutation; MGI; MGI:96680; Kras; Simple; p.Gly12Asp (c.35G>A); Zygosity=Homozygous (from parent cell line).
CC   Genetic integration: Method=Transduction; Gene=FPbase; R9NL8; eGFP (Note=Enhanced GFP).
CC   Genetic integration: Method=Transduction; Gene=MGI; MGI:2149330; Slc5a5.
CC   Genetic integration: Method=Transduction; Gene=UniProtKB; P13249; S.alboniger pac (PuroR).
CC   Genetic integration: Method=Transduction; Gene=UniProtKB; P00552; Transposon Tn5 neo.
CC   Transformant: ChEBI; CHEBI_82373; N-nitroso-N-methylurethane (NNMU).
CC   Derived from site: In situ; Colon; UBERON=UBERON_0001155.
CC   Breed/subspecies: BALB/c.
DI   NCIt; C120044; Mouse colon adenocarcinoma
OX   NCBI_TaxID=10090; ! Mus musculus (Mouse)
HI   CVCL_7256 ! CT26.WT
SX   Female
CA   Cancer cell line
DT   Created: 05-03-18; Last updated: 10-04-25; Version: 12
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