ID   B16F10-mNIS-Neo/eGFP-Puro
AC   CVCL_QZ93
DR   Imanis; CL067
DR   Wikidata; Q54752476
CC   Characteristics: Reporter cell line, transfected with both the LV-mNIS-P2A-Neo and LV-eGFP-PGK-Puro vectors. LV-mNIS-P2A-Neo encodes for mouse Slc5a5 linked via a P2A cleavage peptide to the neo gene under control of the spleen focus-forming virus (SFFV) promoter. LV-eGFP-PGK-Puro encodes for eGFP under control of the spleen focus-forming virus (SFFV) promoter and the pac gene under the control of the mouse PGK promoter.
CC   Transfected with: MGI; MGI:2149330; Slc5a5.
CC   Transfected with: UniProtKB; P42212; GFP (with p.Phe64Leu, p.Ser65Thr and p.His231Leu = EGFP).
CC   Transfected with: UniProtKB; P00552; Transposon Tn5 neo.
CC   Transfected with: UniProtKB; P13249; S.alboniger pac (PuroR).
CC   Derived from site: In situ; Skin; UBERON=UBERON_0002097.
CC   Breed/subspecies: C57BL/6.
DI   NCIt; C21790; Mouse melanoma
OX   NCBI_TaxID=10090; ! Mus musculus (Mouse)
HI   CVCL_0159 ! B16-F10
SX   Male
CA   Cancer cell line
DT   Created: 05-03-18; Last updated: 29-06-23; Version: 7
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