ID   B16F10-Fluc-Neo/mNIS-Puro
AC   CVCL_QZ89
DR   Imanis; CL069
DR   Wikidata; Q54752468
CC   Characteristics: Reporter cell line, transduced with both the LV-Fluc-P2A-Neo and LV-mNIS-PGKp-Puro vectors. LV-Fluc-P2A-Neo encodes for luciferase linked via a P2A cleavage peptide to the neo gene and under control of the spleen focus-forming virus (SFFV) promoter. LV-mNIS-PGKp-Puro encodes for mouse Slc5a5 under control of the spleen focus-forming virus (SFFV) promoter and the PuroR gene under the control of the mouse PGK promoter (Imanis=CL069).
CC   Genetic integration: Method=Transduction; Gene=MGI; MGI:2149330; Slc5a5.
CC   Genetic integration: Method=Transduction; Gene=UniProtKB; P00552; Klebsiella pneumoniae transposon Tn5 neo.
CC   Genetic integration: Method=Transduction; Gene=UniProtKB; P08659; Photinus pyralis firefly-type luciferase.
CC   Genetic integration: Method=Transduction; Gene=UniProtKB; P13249; Streptomyces alboniger pac (PuroR).
CC   Derived from site: In situ; Skin; UBERON=UBERON_0002097.
CC   Breed/subspecies: C57BL/6.
DI   NCIt; C21790; Mouse melanoma
OX   NCBI_TaxID=10090; ! Mus musculus (Mouse)
HI   CVCL_0159 ! B16-F10
SX   Male
AG   Age unspecified
CA   Cancer cell line
DT   Created: 05-03-18; Last updated: 14-08-25; Version: 12
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