ID   A375-iRFP-Neo/Fluc-Puro
AC   CVCL_QZ74
DR   cancercelllines; CVCL_QZ74
DR   Imanis; CL089
DR   Wikidata; Q54606795
CC   Population: Caucasian.
CC   Characteristics: Reporter cell line, transfected with both the LV-iRFP-P2A-Neo and LV-Fluc-P2A-Puro vectors. LV-iRFP-P2A-Neo encodes for iRFP linked via a P2A cleavage peptide to the neo gene and under control of the spleen focus-forming virus (SFFV) promoter. LV-Fluc-P2A-Puro encodes for luciferase linked via a P2A cleavage peptide to the pac gene and under control of the spleen focus-forming virus (SFFV) promoter.
CC   Sequence variation: Mutation; HGNC; 1097; BRAF; Simple; p.Val600Glu (c.1799T>A); ClinVar=VCV000013961; Zygosity=Homozygous (from parent cell line).
CC   Sequence variation: Mutation; HGNC; 1787; CDKN2A; Simple; p.Glu61Ter (c.181G>T) (p.Gly75Val, c.224G>T); ClinVar=VCV000376309; Zygosity=Homozygous (from parent cell line).
CC   Sequence variation: Mutation; HGNC; 1787; CDKN2A; Simple; p.Glu69Ter (c.205G>T) (p.Gly83Val, c.248G>T); ClinVar=VCV000376308; Zygosity=Homozygous (from parent cell line).
CC   Sequence variation: Mutation; HGNC; 11730; TERT; Simple; c.1-146C>T (c.250C>T) (C250T); Zygosity=Unspecified; Note=In promoter (from parent cell line).
CC   Transfected with: UniProtKB; P08659; Firefly luciferase.
CC   Transfected with: UniProtKB; G1FNL7; Rhodopseudomonas palustris iRFP.
CC   Transfected with: UniProtKB; P00552; Transposon Tn5 neo.
CC   Transfected with: UniProtKB; P13249; S.alboniger pac (PuroR).
CC   Derived from site: In situ; Skin; UBERON=UBERON_0002097.
DI   NCIt; C3802; Amelanotic melanoma
OX   NCBI_TaxID=9606; ! Homo sapiens (Human)
HI   CVCL_0132 ! A-375
SX   Female
AG   54Y
CA   Cancer cell line
DT   Created: 05-03-18; Last updated: 05-10-23; Version: 12
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