ID   4T1-mNIS-Puro/Fluc-Neo
AC   CVCL_QZ65
SY   4T1-Fluc-Neo/mNIS-Puro
DR   Imanis; CL066
DR   Wikidata; Q54603553
RX   PubMed=30674994;
CC   Characteristics: Reporter cell line, transfected with both the LV-mNIS-PGK-Puro and LV-Fluc-P2A-Neo vectors. LV-mNIS-PGK-Puro encodes for mouse Slc5a5 under control of the spleen focus-forming virus (SFFV) promoter and the pac gene under the control of the mouse PGK promoter. LV-Fluc-P2A-Neo encodes for luciferase linked via a P2A cleavage peptide to the neo gene and under control of the spleen focus-forming virus (SFFV) promoter (Imanis=CL066).
CC   Selected for resistance to: ChEBI; CHEBI:9555; Tioguanine (6-thioguanine; 6-TG).
CC   Transfected with: MGI; MGI:2149330; Slc5a5.
CC   Transfected with: UniProtKB; P08659; Firefly luciferase.
CC   Transfected with: UniProtKB; P00552; Transposon Tn5 neo.
CC   Transfected with: UniProtKB; P13249; S.alboniger pac (PuroR).
CC   Derived from site: In situ; Mammary gland; UBERON=UBERON_0001911.
CC   Breed/subspecies: BALB/cfC3H.
DI   NCIt; C21678; Malignant neoplasms of the mouse mammary gland
OX   NCBI_TaxID=10090; ! Mus musculus (Mouse)
HI   CVCL_0125 ! 4T1
SX   Female
CA   Cancer cell line
DT   Created: 05-03-18; Last updated: 02-05-24; Version: 10
//
RX   PubMed=30674994; DOI=10.1038/s41417-019-0081-2;
RA   Vandergaast R., Khongwichit S., Jiang H.-L., DeGrado T.R., Peng K.-W.,
RA   Smith D.R., Russell S.J., Suksanpaisan L.;
RT   "Enhanced noninvasive imaging of oncology models using the NIS
RT   reporter gene and bioluminescence imaging.";
RL   Cancer Gene Ther. 27:179-188(2020).
//