ID   WI-38/hTERT/GFP-RAF-ER
AC   CVCL_N806
DR   ENCODE; ENCBS070MZP
DR   ENCODE; ENCBS157XHV
DR   ENCODE; ENCBS601ENJ
DR   ENCODE; ENCBS606ENC
DR   ENCODE; ENCBS607ENC
DR   ENCODE; ENCBS608ENC
DR   ENCODE; ENCBS715PYA
DR   ENCODE; ENCBS787LHJ
DR   GEO; GSM651580
DR   GEO; GSM651581
DR   GEO; GSM651582
DR   GEO; GSM651583
DR   GEO; GSM945215
DR   GEO; GSM945223
DR   GEO; GSM945245
DR   GEO; GSM945265
DR   Wikidata; Q54994036
WW   Info; ENCODE; -; http://genome.ucsc.edu/ENCODE/protocols/cell/human/WI38_Stam_protocol.pdf
CC   Part of: ENCODE project common cell types; tier 3.
CC   Population: Caucasian; Swedish.
CC   Characteristics: Transduced with a construct that is composed of EGFP at the N-terminus, the catalytic domain of RAF1 (with p.Tyr340Asp and p.Tyr341Asp) in the middle and the hormone-binding domain of ESR1 at the C-terminus. Protein kinase activity is activated by estrogen or analogs such as 4-hydroxy-tamoxifen.
CC   Senescence: Cells can be senesced after exposure for 72 hours with 4-hydroxy-tamoxifen.
CC   Genetic integration: Method=Transduction; Gene=FPbase; R9NL8; eGFP (Note=Enhanced GFP).
CC   Genetic integration: Method=Transduction; Gene=HGNC; HGNC:3467; ESR1 (Note=Hormone-binding domain).
CC   Genetic integration: Method=Transduction; Gene=HGNC; HGNC:9829; RAF1 (Note=Catalytic domain with p.Tyr340Asp and p.Tyr341Asp).
CC   Genetic integration: Method=Transduction; Gene=HGNC; HGNC:11730; TERT.
CC   Genetic integration: Method=Transduction; Gene=UniProtKB; P00552; Klebsiella pneumoniae transposon Tn5 neo.
CC   Genetic integration: Method=Transduction; Gene=UniProtKB; P13249; Streptomyces alboniger pac (PuroR).
CC   Omics: Genomics; ChIP-seq; H3K4me3.
CC   Derived from site: In situ; Fetal lung; UBERON=UBERON_0005597.
CC   Cell type: Fibroblast of lung; CL=CL_0002553.
OX   NCBI_TaxID=9606; ! Homo sapiens (Human)
HI   CVCL_0579 ! WI-38
SX   Female
AG   16FW
CA   Telomerase immortalized cell line
DT   Created: 05-11-13; Last updated: 14-08-25; Version: 19
//