ID   A549 VIM RFP
AC   CVCL_LI35
SY   A549 Vim RFP; A-549 VIM RFP
DR   ATCC; CCL-185EMT
DR   cancercelllines; CVCL_LI35
DR   Wikidata; Q54607012
CC   Population: Caucasian.
CC   Characteristics: Using CRISPR/Cas9 a red fluorescent protein (RFP) tag was added to the C-terminal of the VIM gene. Allows to monitor the epithelial-to-mesenchymal transition (EMT) status of cells in vitro by monitoring RFP expression.
CC   Sequence variation: Mutation; HGNC; 6407; KRAS; Simple; p.Gly12Ser (c.34G>A); ClinVar=VCV000012584; Zygosity=Homozygous (from parent cell line).
CC   Sequence variation: Mutation; HGNC; 11389; STK11; Simple; p.Gln37Ter (c.109C>T); ClinVar=VCV000376334; Zygosity=Homozygous (from parent cell line).
CC   Transfected with: UniProtKB; Q9U6Y8; Discosoma sp. red fluorescent protein drFP583 (DsRed, RFP).
CC   Derived from site: In situ; Lung; UBERON=UBERON_0002048.
ST   Source(s): ATCC=CCL-185EMT
ST   Amelogenin: X,Y
ST   CSF1PO: 10,12
ST   D13S317: 11
ST   D16S539: 11,12
ST   D5S818: 11
ST   D7S820: 8,11
ST   TH01: 8,9.3
ST   TPOX: 8,11
ST   vWA: 14
DI   NCIt; C3512; Lung adenocarcinoma
OX   NCBI_TaxID=9606; ! Homo sapiens (Human)
HI   CVCL_0023 ! A-549
SX   Male
AG   58Y
CA   Cancer cell line
DT   Created: 15-11-17; Last updated: 02-05-24; Version: 12
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