ID   GeneBLAzer MR-UAS-bla HEK 293T
AC   CVCL_LF47
SY   MR-UAS-bla HEK 293T
DR   Wikidata; Q54835706
WW   Provider; Thermo Fisher; K1696; https://www.thermofisher.com/order/catalog/product/K1696
CC   Characteristics: Transfected with a plasmid containing a beta-lactamase reporter gene under control of an upstream activator sequence (UAS).
CC   Characteristics: Transfected with the ligand-binding domain (LBD) of NR3C2/MR fused to the DNA-binding domain (DBD) of GAL4. When an agonist binds to the LBD of the DBD-LBD fusion protein, the protein binds to the UAS, resulting in expression of the beta-lactamase.
CC   Genetic integration: Method=Transfection; Gene=HGNC; HGNC:7979; NR3C2 (Note=Ligand-binding domain).
CC   Genetic integration: Method=Transfection; Gene=UniProtKB; P33967; B.cereus Bsr.
CC   Genetic integration: Method=Transfection; Gene=UniProtKB; P62593; Escherichia coli beta-lactamase bla (Note=With p.Met1_Phe22del, p.Ala23Met, p.Val82Ile and p.Met184Val).
CC   Genetic integration: Method=Transfection; Gene=UniProtKB; P03070; SV40 large T antigen.
CC   Genetic integration: Method=Transfection; Gene=UniProtKB; P00552; Transposon Tn5 neo.
CC   Genetic integration: Method=Transfection; Gene=UniProtKB; P04386; Yeast GAL4 (Note=With p.Ile148_Glu881del = DNA-binding domain).
CC   Transformant: NCBI_TaxID; 28285; Adenovirus 5.
CC   Transformant: NCBI_TaxID; 1891767; Simian virus 40 (SV40) (Note=tsA).
CC   Derived from site: In situ; Fetal kidney; UBERON=UBERON_0002113.
OX   NCBI_TaxID=9606; ! Homo sapiens (Human)
HI   CVCL_LF40 ! GeneBLAzer UAS-bla HEK 293T
SX   Female
AG   Fetus
CA   Transformed cell line
DT   Created: 22-08-17; Last updated: 10-04-25; Version: 13
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