ID   NAKT-15
AC   CVCL_L910
SY   NAKT15
DR   Wikidata; Q54907375
RX   PubMed=16186810;
RX   PubMed=16211061;
CC   Characteristics: Secretes insulin in response to glucose stimulation and nonglucose secretagogues, and expresses proteins characteristic of beta-cells.
CC   Characteristics: Because it has been transduced by two constructs containing LoxP sites, transfection with the Cre recombinase eliminates both the SV40 T gene and TERT thus reverting the cells to a non-immortal phenotype.
CC   Characteristics: Transduced with two retroviral vectors: 1) SSR#197 (MoMLV/LTR-LoxP-hTERT-IRES-EGFP-LoxP-MoMLV/LTR) expressing hTERT and eGFP flanked by two loxP sites and 2) SSR#69 (MoMLV/LTR-LoxP-HygroR-HSV/TK-IRES-SV40T-LoxP-Neo-MoMLV/LTR) expressing HygroR, HSV-1 TK and SV40 T-antigen flanked by two loxP sites.
CC   Genetic integration: Method=Transduction; Gene=FPbase; R9NL8; eGFP (Note=Enhanced GFP).
CC   Genetic integration: Method=Transduction; Gene=HGNC; HGNC:11730; TERT.
CC   Genetic integration: Method=Transduction; Gene=UniProtKB; P00557; Escherichia coli hygromycin-B 4-O-kinase (hph) (HygR).
CC   Genetic integration: Method=Transduction; Gene=UniProtKB; Q9QNF7; Human herpesvirus 1 TK.
CC   Genetic integration: Method=Transduction; Gene=UniProtKB; P00552; Transposon Tn5 neo.
CC   Transformant: NCBI_TaxID; 1891767; Simian virus 40 (SV40).
CC   Derived from site: In situ; Pancreas, islets of Langerhans; UBERON=UBERON_0000006.
OX   NCBI_TaxID=9606; ! Homo sapiens (Human)
SX   Sex unspecified
AG   Age unspecified
CA   Transformed cell line
DT   Created: 06-05-13; Last updated: 10-04-25; Version: 15
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RX   PubMed=16186810; DOI=10.1038/nbt1145;
RA   Narushima M., Kobayashi N., Okitsu T., Tanaka Y., Li S.-A., Chen Y.,
RA   Miki A., Tanaka K., Nakaji S., Takei K., Gutierrez A.S.,
RA   Rivas-Carrillo J.D., Navarro-Alvarez N., Jun H.-S., Westerman K.A.,
RA   Noguchi H., Lakey J.R.T., Leboulch P., Tanaka N., Yoon J.-W.;
RT   "A human beta-cell line for transplantation therapy to control type 1
RT   diabetes.";
RL   Nat. Biotechnol. 23:1274-1282(2005).
//
RX   PubMed=16211061; DOI=10.1038/nbt1005-1231;
RA   Hohmeier H.-E., Newgard C.B.;
RT   "Islets for all?";
RL   Nat. Biotechnol. 23:1231-1232(2005).
//