ID   PathHunter HEK 293 ADRB2 Total GPCR Internalization
AC   CVCL_KZ32
SY   PathHunter ADRB2 Total GPCR Internalization HEK 293
DR   DiscoverX; 93-0800C1
DR   Wikidata; Q54938010
CC   Characteristics: PathHunter total GPCR internalization cell lines co-express a tagged with a beta-galactosidase fragment (ProLink=PK) and a second beta-galactosidase fragment (Enzyme Acceptor=EA) localized to the endosomes. Activation of the GPCR-PK induces internalization of the receptor in EA-tagged endosomes and complementation of the PK and EA enzyme fragments thus resulting into a catalytically active enzyme that generate a chemiluminescent signal.
CC   Genetic integration: Method=Transfection; Gene=HGNC; HGNC:286; ADRB2.
CC   Transformant: NCBI_TaxID; 28285; Human adenovirus C serotype 5.
CC   Derived from site: In situ; Fetal kidney; UBERON=UBERON_0002113.
OX   NCBI_TaxID=9606; ! Homo sapiens (Human)
HI   CVCL_0045 ! HEK293
SX   Female
AG   Fetus
CA   Transformed cell line
DT   Created: 22-08-17; Last updated: 14-08-25; Version: 9
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