ID   MS705
AC   CVCL_KA71
DR   Wikidata; Q54906809
RX   PubMed=15352059;
CC   Characteristics: Useful for the production of fucose-negative antibodies.
CC   Knockout cell: Method=Homologous recombination; UniProtKB; G3HCE4; Fut8.
CC   Selected for resistance to: ChEBI; CHEBI_44185; Methotrexate (MTX).
CC   Sequence variation: Gene deletion; UniProtKB; Q2MH30; Dhfr; Zygosity=Homozygous (from parent cell line).
CC   Transformant: NCIt; C129640; Cobalt-60 gamma radiation.
CC   Derived from site: In situ; Ovary; UBERON=UBERON_0000992.
CC   Cell type: Epithelial cell of ovary; CL=CL_2000064.
OX   NCBI_TaxID=10029; ! Cricetulus griseus (Chinese hamster)
HI   CVCL_7180 ! CHO-DG44
SX   Female
AG   Adult
CA   Transformed cell line
DT   Created: 22-08-17; Last updated: 19-12-24; Version: 10
//
RX   PubMed=15352059; DOI=10.1002/bit.20151;
RA   Yamane-Ohnuki, Naoko
RA   Kinoshita, Satoko
RA   Inoue-Urakubo, Miho
RA   Kusunoki, Machi
RA   Iida, Shigeru
RA   Nakano, Ryosuke
RA   Wakitani, Masako
RA   Niwa, Rinpei
RA   Sakurada, Mikiko
RA   Uchida, Kazuhisa
RA   Shitara, Kenya
RA   Satoh, Mitsuo
RT   "Establishment of FUT8 knockout Chinese hamster ovary cells: an ideal
RT   host cell line for producing completely defucosylated antibodies with
RT   enhanced antibody-dependent cellular cytotoxicity.";
RL   Biotechnol. Bioeng. 87:614-622(2004).
//