ID   MS705
AC   CVCL_KA71
DR   Wikidata; Q54906809
RX   PubMed=15352059;
CC   Characteristics: Useful for the production of fucose-negative antibodies.
CC   Characteristics: Lacks both copies of the Dhfr gene.
CC   Knockout cell: Method=Homologous recombination; UniProtKB; G3HCE4; Fut8.
CC   Selected for resistance to: ChEBI; CHEBI:44185; Methotrexate (MTX).
CC   Transformant: NCIt; C129640; Cobalt-60 gamma radiation.
CC   Derived from site: In situ; Ovary; UBERON=UBERON_0000992.
CC   Cell type: Epithelial cell of ovary; CL=CL_2000064.
OX   NCBI_TaxID=10029; ! Cricetulus griseus (Chinese hamster)
HI   CVCL_7180 ! CHO-DG44
SX   Female
AG   Adult
CA   Spontaneously immortalized cell line
DT   Created: 22-08-17; Last updated: 30-01-24; Version: 9
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RX   PubMed=15352059; DOI=10.1002/bit.20151;
RA   Yamane-Ohnuki N., Kinoshita S., Inoue-Urakubo M., Kusunoki M.,
RA   Iida S., Nakano R., Wakitani M., Niwa R., Sakurada M., Uchida K.,
RA   Shitara K., Satoh M.;
RT   "Establishment of FUT8 knockout Chinese hamster ovary cells: an ideal
RT   host cell line for producing completely defucosylated antibodies with
RT   enhanced antibody-dependent cellular cytotoxicity.";
RL   Biotechnol. Bioeng. 87:614-622(2004).
//