ID   Ms704
AC   CVCL_KA70
SY   MS704
DR   Wikidata; Q54906807
RX   PubMed=15352059;
RX   PubMed=16609957;
CC   Characteristics: Useful for the production of fucose-negative antibodies.
CC   Knockout cell: Method=Homologous recombination; UniProtKB; G3HCE4; Fut8.
CC   Selected for resistance to: ChEBI; CHEBI_44185; Methotrexate (MTX).
CC   Sequence variation: Gene deletion; UniProtKB; Q2MH30; Dhfr; Zygosity=Homozygous (from parent cell line).
CC   Transformant: NCIt; C129640; Cobalt-60 gamma radiation.
CC   Derived from site: In situ; Ovary; UBERON=UBERON_0000992.
CC   Cell type: Epithelial cell of ovary; CL=CL_2000064.
OX   NCBI_TaxID=10029; ! Cricetulus griseus (Chinese hamster)
HI   CVCL_7180 ! CHO-DG44
SX   Female
AG   Adult
CA   Transformed cell line
DT   Created: 22-08-17; Last updated: 19-12-24; Version: 10
//
RX   PubMed=15352059; DOI=10.1002/bit.20151;
RA   Yamane-Ohnuki N., Kinoshita S., Inoue-Urakubo M., Kusunoki M.,
RA   Iida S., Nakano R., Wakitani M., Niwa R., Sakurada M., Uchida K.,
RA   Shitara K., Satoh M.;
RT   "Establishment of FUT8 knockout Chinese hamster ovary cells: an ideal
RT   host cell line for producing completely defucosylated antibodies with
RT   enhanced antibody-dependent cellular cytotoxicity.";
RL   Biotechnol. Bioeng. 87:614-622(2004).
//
RX   PubMed=16609957; DOI=10.1002/bit.20880;
RA   Kanda Y., Yamane-Ohnuki N., Sakai N., Yamano K., Nakano R.,
RA   Inoue-Urakubo M., Misaka H., Iida S., Wakitani M., Konno Y., Yano K.,
RA   Shitara K., Hosoi S., Satoh M.;
RT   "Comparison of cell lines for stable production of fucose-negative
RT   antibodies with enhanced ADCC.";
RL   Biotechnol. Bioeng. 94:680-688(2006).
//