ID   HeLa-X4
AC   CVCL_JL48
SY   HeLa-X4-6
DR   Wikidata; Q54882735
RX   PubMed=16314403;
CC   Population: African American.
CC   Characteristics: Engineered to express the gp160 envelope protein (Env) from a CXCR4-tropic HIV-1 isolate as well as a Tat-GFP fusion protein (PubMed=16314403).
CC   Characteristics: Transfected with reverse tetracycline-controlled transactivator (rtTA), a fusion between a mutated version of E.coli TetR and the activating domain of HSV-1 VP16 (from parent cell line).
CC   Genetic integration: Method=Transfection; Gene=FPbase; 1XF1B; GFP (Note=avGFP; original A.victoria green fluorescent protein).
CC   Genetic integration: Method=Transfection; Gene=UniProtKB; P00557; Escherichia coli hygromycin-B 4-O-kinase (hph) (HygR).
CC   Genetic integration: Method=Transfection; Gene=UniProtKB; P04610; HIV-1 isolate BRU/LAI tat.
CC   Genetic integration: Method=Transfection; Gene=UniProtKB; Q72502; HIV-1 isolate NL4-3 env.
CC   Genetic integration: Method=Transfection/transduction; Gene=UniProtKB; P04483; Escherichia coli Tn10 tetR.
CC   Genetic integration: Method=Transfection/transduction; Gene=UniProtKB; P06492; Human herpesvirus 1 VP16 (UL28) (Note=With p.Met1_Arg360del).
CC   Transformant: NCBI_TaxID; 333761; Human papillomavirus type 18 (HPV18).
CC   Derived from site: In situ; Uterus, cervix; UBERON=UBERON_0000002.
DI   NCIt; C27677; Human papillomavirus-related endocervical adenocarcinoma
OX   NCBI_TaxID=9606; ! Homo sapiens (Human)
HI   CVCL_IY74 ! HeLa Tet-On
SX   Female
AG   30Y6M
CA   Cancer cell line
DT   Created: 15-05-17; Last updated: 10-04-25; Version: 13
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RX   PubMed=16314403; DOI=10.1177/1087057105282959;
RA   Ji C.-H., Zhang J., Cammack N., Sankuratri S.;
RT   "Development of a novel dual CCR5-dependent and CXCR4-dependent
RT   cell-cell fusion assay system with inducible gp160 expression.";
RL   J. Biomol. Screen. 11:65-74(2006).
//