ID   Hep-2020
AC   CVCL_JG90
SY   Hep2020
DR   JCRB; JCRB1699
DR   Wikidata; Q54882759
RX   PubMed=33060611;
CC   Characteristics: The CCDN1, CDK4 and TERT genes expression can be silenced by administration of tetracycline/doxycycline through a Tet-Off system (PubMed=33060611).
CC   Genetic integration: Method=Transduction; Gene=HGNC; HGNC:1582; CCND1.
CC   Genetic integration: Method=Transduction; Gene=HGNC; HGNC:1773; CDK4 (Note=With p.Arg24Cys).
CC   Genetic integration: Method=Transduction; Gene=HGNC; HGNC:11730; TERT.
CC   Genetic integration: Method=Transduction; Gene=UniProtKB; P04483; Escherichia coli Tn10 tetR.
CC   Genetic integration: Method=Transduction; Gene=UniProtKB; P06492; Human herpesvirus 1 VP16 (UL28) (Note=With p.Met1_Arg360del).
CC   Derived from site: In situ; Liver; UBERON=UBERON_0002107.
CC   Cell type: Hepatocyte; CL=CL_0000182.
ST   Source(s): JCRB=JCRB1699
ST   Amelogenin: X,Y
ST   CSF1PO: 12
ST   D13S317: 11
ST   D16S539: 9,11
ST   D5S818: 13
ST   D7S820: 12
ST   TH01: 9
ST   TPOX: 8
ST   vWA: 14,17
DI   NCIt; C97071; Congenital hepatic fibrosis
DI   ORDO; Orphanet_485426; Isolated congenital hepatic fibrosis
OX   NCBI_TaxID=9606; ! Homo sapiens (Human)
SX   Male
AG   6Y
CA   Telomerase immortalized cell line
DT   Created: 15-05-17; Last updated: 19-12-24; Version: 13
//
RX   PubMed=33060611; DOI=10.1038/s41598-020-73992-3; PMCID=PMC7567112;
RA   Nishiwaki M., Toyoda M., Oishi Y., Ishida S., Horiuchi S.-i.,
RA   Makino-Itou H., Kimura T., Ohno S.-i., Ohkura T., Enosawa S.,
RA   Akutsu H., Nakazawa A., Kasahara M., Kiyono T., Umezawa A.;
RT   "Immortalization of human hepatocytes from biliary atresia with
RT   CDK4(R24C), cyclin D1, and TERT for cytochrome P450 induction
RT   testing.";
RL   Sci. Rep. 10:17503.1-17503.13(2020).
//