ID   CHO-K1 tsTM4
AC   CVCL_J617
SY   tsTM4
DR   Wikidata; Q54973166
RX   PubMed=2237640;
RX   PubMed=9272869;
CC   Characteristics: Temperature sensitive. Exhibits abnormal induction of sister chromatid exchanges along with a decrease of DNA synthesis in the cells arrested in S phase at the non-permissive temperature of 39 Celsius (PubMed=9272869).
CC   Doubling time: 34 hours (PubMed=2237640).
CC   Sequence variation: Mutation; UniProtKB; P11414; Polr2a; Simple; p.Pro1006Ser (c.3016C>T); Zygosity=Homozygous (PubMed=9272869).
CC   Transformant: ChEBI; CHEBI:21759; N-methyl-N'-nitro-N-nitrosoguanidine (MNNG).
CC   Derived from site: In situ; Ovary; UBERON=UBERON_0000992.
CC   Cell type: Epithelial cell of ovary; CL=CL_2000064.
OX   NCBI_TaxID=10029; ! Cricetulus griseus (Chinese hamster)
HI   CVCL_0214 ! CHO-K1
SX   Female
AG   Adult
CA   Transformed cell line
DT   Created: 11-02-13; Last updated: 30-01-24; Version: 10
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RX   PubMed=2237640; DOI=10.1007/BF01233196;
RA   Tsuji H., Matsudo Y., Tsuji S., Hanaoka F., Hyodo M., Hori T.-a.;
RT   "Isolation of temperature-sensitive CHO-K1 cell mutants exhibiting
RT   chromosomal instability and reduced DNA synthesis at nonpermissive
RT   temperature.";
RL   Somat. Cell Mol. Genet. 16:461-476(1990).
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RX   PubMed=9272869; DOI=10.1016/S0378-1119(97)00204-7;
RA   Sugaya K., Sasanuma S.-i., Nohata J., Kimura T., Hongo E., Higashi T.,
RA   Morimyo M., Tsuji H., Mita K.;
RT   "Cloning and sequencing for the largest subunit of Chinese hamster RNA
RT   polymerase II gene: identification of a mutation related to abnormal
RT   induction of sister chromatid exchanges.";
RL   Gene 194:267-272(1997).
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