ID   WTC-mEGFP-LMNB1-cl210
AC   CVCL_IR32
SY   AICS-0013-210; AICS0013; AICS-0013; LMNB1 cl. 210; AICSi002-A-6; UCSFi001-A-6
DR   BioSamples; SAMEA6306320
DR   Coriell; AICS-0013
DR   hPSCreg; UCSFi001-A-6
DR   Wikidata; Q54994545
RX   PubMed=28814507;
RX   PubMed=30199041;
WW   Info; Allen Institute; -; https://www.allencell.org/cell-catalog.html
WW   Info; Allen Institute; -; https://www.allencell.org/genomics.html
CC   Part of: Allen Cell Collection.
CC   Population: Japanese.
CC   Characteristics: Using CRISPR/Cas9 LMNB1 has been endogenously tagged in one allele at the N-terminus with mEGFP.
CC   Genetic integration: Method=CRISPR/Cas9; Gene=FPbase; QKFJN; mEGFP (Note=Enhanced GFP).
CC   Omics: Genomics; Whole exome sequencing.
CC   Derived from site: In situ; Leg, skin; UBERON=UBERON_0001511.
CC   Cell type: Fibroblast of skin; CL=CL_0002620.
OX   NCBI_TaxID=9606; ! Homo sapiens (Human)
HI   CVCL_Y803 ! GM25256
SX   Male
AG   30Y
CA   Induced pluripotent stem cell
DT   Created: 03-03-17; Last updated: 10-04-25; Version: 16
//
RX   PubMed=28814507; DOI=10.1091/mbc.E17-03-0209; PMCID=PMC5638588;
RA   Roberts, Brock
RA   Haupt, Amanda
RA   Tucker, Andrew
RA   Grancharova, Tanya
RA   Arakaki, Joy
RA   Fuqua, Margaret A.
RA   Nelson, Angelique M.
RA   Hookway, Caroline
RA   Ludmann, Susan A.
RA   Mueller, Irina A.
RA   Yang, Rui-An
RA   Horwitz, Rick
RA   Rafelski, Susanne Marie
RA   Gunawardane, Ruwanthi N.
RT   "Systematic gene tagging using CRISPR/Cas9 in human stem cells to
RT   illuminate cell organization.";
RL   Mol. Biol. Cell 28:2854-2874(2017).
//
RX   PubMed=30199041; DOI=10.3791/58130; PMCID=PMC6231893;
RA   Haupt, Amanda
RA   Grancharova, Tanya
RA   Arakaki, Joy
RA   Fuqua, Margaret A.
RA   Roberts, Brock
RA   Gunawardane, Ruwanthi N.
RT   "Endogenous protein tagging in human induced pluripotent stem cells
RT   using CRISPR/Cas9.";
RL   J. Vis. Exp. 138:e58130.1-e58130.9(2018).
//