ID   RKO BRAF(V600E/-/-)
AC   CVCL_HE17
SY   BRAF (V600E/-/-) RKO
DR   cancercelllines; CVCL_HE17
DR   Horizon_Discovery; HD+106-004
DR   Wikidata; Q54950541
RX   PubMed=19661383;
CC   Characteristics: RKO carries a stable triplication of the BRAF gene with one wild-type (V-600) and two mutant alleles (E-600). This KO cell line carries only the mutant allele.
CC   Knockout cell: Method=Homologous recombination; HGNC; HGNC:1097; BRAF (Note=2 of 3 alleles).
CC   Sequence variation: Mutation; HGNC; HGNC:173; ACVR2A; Simple; p.Lys437Argfs*5 (c.1310delA); dbSNP=rs764719749; Zygosity=Heterozygous (from parent cell line).
CC   Sequence variation: Mutation; HGNC; HGNC:1097; BRAF; Simple_edited; p.Val600Glu (c.1799T>A); ClinVar=VCV000013961; Zygosity=Homozygous; Note=By method not specified (Horizon_Discovery=HD+106-004).
CC   Sequence variation: Mutation; HGNC; HGNC:8975; PIK3CA; Simple; p.His1047Arg (c.3140A>G); ClinVar=VCV000013652; Zygosity=Heterozygous (from parent cell line).
CC   Sequence variation: Mutation; HGNC; HGNC:11773; TGFBR2; Simple; p.Lys128Serfs*35 (c.383delA); ClinVar=VCV000477546; Zygosity=Heterozygous (from parent cell line).
CC   Sequence variation: Mutation; HGNC; HGNC:11773; TGFBR2; Simple; p.Leu452Pro (c.1355T>C); ClinVar=VCV000930524; Zygosity=Heterozygous (from parent cell line).
CC   Derived from site: In situ; Colon; UBERON=UBERON_0001155.
DI   NCIt; C4910; Colon carcinoma
OX   NCBI_TaxID=9606; ! Homo sapiens (Human)
HI   CVCL_0504 ! RKO
SX   Sex unspecified
AG   Age unspecified
CA   Cancer cell line
DT   Created: 01-12-16; Last updated: 19-12-24; Version: 13
//
RX   PubMed=19661383; DOI=10.1126/science.1174229; PMCID=PMC2820374;
RA   Yun, Jiyeon
RA   Rago, Carlo
RA   Cheong, Ian
RA   Pagliarini, Raymond
RA   Angenendt, Philipp
RA   Rajagopalan, Harith
RA   Schmidt, Kerstin
RA   Willson, James K.V.
RA   Markowitz, Sanford David
RA   Zhou, Shi-Bin
RA   Diaz, Luis Alberto Jr.
RA   Velculescu, Victor E.
RA   Lengauer, Christoph
RA   Kinzler, Kenneth Wayne
RA   Vogelstein, Bert
RA   Papadopoulos, Nickolas
RT   "Glucose deprivation contributes to the development of KRAS pathway
RT   mutations in tumor cells.";
RL   Science 325:1555-1559(2009).
//