ID   HCT 116 CDKN1A(-/-)
AC   CVCL_HD73
SY   CDKN1A (-/-) HCT116; HCT116 p21-/-; 80S14
DR   BTO; BTO:0006532
DR   cancercelllines; CVCL_HD73
DR   Horizon_Discovery; HD+R02-035
DR   Wikidata; Q54881926
RX   PubMed=7585571;
RX   PubMed=8756351;
CC   Population: Caucasian.
CC   Knockout cell: Method=Homologous recombination; HGNC; 1784; CDKN1A.
CC   Sequence variation: Mutation; HGNC; 173; ACVR2A; Simple; p.Lys437Argfs*5 (c.1310delA); dbSNP=rs764719749; Zygosity=Homozygous (from parent cell line).
CC   Sequence variation: Mutation; HGNC; 1101; BRCA2; Simple; p.Ile2675Aspfs*6 (c.8021dupA) (c.8021_8022insA); ClinVar=VCV000267050; Zygosity=Heterozygous (from parent cell line).
CC   Sequence variation: Mutation; HGNC; 1787; CDKN2A; Simple; p.Arg24Serfs*20 (c.68dupG) (c.68_69insG) (p.G23fs); Zygosity=Heterozygous (from parent cell line).
CC   Sequence variation: Mutation; HGNC; 2514; CTNNB1; Simple; p.Ser45del (c.133_135delTCT); ClinVar=VCV000017576; Zygosity=Heterozygous (from parent cell line).
CC   Sequence variation: Mutation; HGNC; 3373; EP300; Simple; p.Met1470Cysfs*22 (c.4408delA); Zygosity=Heterozygous (from parent cell line).
CC   Sequence variation: Mutation; HGNC; 3373; EP300; Simple; p.Asn1700Thrfs*9 (c.5099delA); Zygosity=Heterozygous (from parent cell line).
CC   Sequence variation: Mutation; HGNC; 6407; KRAS; Simple; p.Gly13Asp (c.38G>A); ClinVar=VCV000012580; Zygosity=Heterozygous (from parent cell line).
CC   Sequence variation: Mutation; HGNC; 8975; PIK3CA; Simple; p.His1047Arg (c.3140A>G); ClinVar=VCV000013652; Zygosity=Heterozygous (from parent cell line).
CC   Sequence variation: Mutation; HGNC; 9277; PPM1D; Simple; p.Leu450Ter (c.1349delT) (p.Leu450fs) (c.1344delT); Zygosity=Heterozygous (from parent cell line).
CC   Sequence variation: Mutation; HGNC; 11773; TGFBR2; Simple; p.Lys128Serfs*35 (c.383delA); ClinVar=VCV000477546; Zygosity=Homozygous (from parent cell line).
CC   Caution: There are two clones of p21-/- cells that were created, 80S4 and 80S14. It is not clear which clone this cell line corresponds to, but as 80S14 was much more used than 80S4 we believe it is that clone.
CC   Derived from site: In situ; Colon; UBERON=UBERON_0001155.
DI   NCIt; C4910; Colon carcinoma
OX   NCBI_TaxID=9606; ! Homo sapiens (Human)
HI   CVCL_0291 ! HCT 116
SX   Male
AG   48Y
CA   Cancer cell line
DT   Created: 01-12-16; Last updated: 05-10-23; Version: 12
//
RX   PubMed=7585571;
RA   Waldman T., Kinzler K.W., Vogelstein B.;
RT   "p21 is necessary for the p53-mediated G1 arrest in human cancer
RT   cells.";
RL   Cancer Res. 55:5187-5190(1995).
//
RX   PubMed=8756351; DOI=10.1101/gad.10.15.1945;
RA   Polyak K., Waldman T., He T.-C., Kinzler K.W., Vogelstein B.;
RT   "Genetic determinants of p53-induced apoptosis and growth arrest.";
RL   Genes Dev. 10:1945-1952(1996).
//