ID   IMR-90/GFPdeltaRaf-1[YY]:ER
AC   CVCL_HA48
DR   Wikidata; Q54897673
RX   PubMed=9765202;
CC   Population: Caucasian.
CC   Characteristics: Transfected with a construct that is composed of EGFP at the N-terminus, the catalytic domain of RAF1 (with p.Tyr340Asp and p.Tyr341Asp) in the middle and the hormone-binding domain of ESR1 at the C-terminus. Protein kinase activity is activated by estrogen or analogs such as 4-hydroxy-tamoxifen.
CC   Genetic integration: Method=Transfection/transduction; Gene=FPbase; R9NL8; eGFP (Note=Enhanced GFP).
CC   Genetic integration: Method=Transfection/transduction; Gene=HGNC; HGNC:3467; ESR1 (Note=Hormone-binding domain).
CC   Genetic integration: Method=Transfection/transduction; Gene=HGNC; HGNC:9829; RAF1 (Note=Catalytic domain with p.Tyr340Asp and p.Tyr341Asp).
CC   Derived from site: In situ; Fetal lung; UBERON=UBERON_0005597.
CC   Cell type: Fibroblast of lung; CL=CL_0002553.
OX   NCBI_TaxID=9606; ! Homo sapiens (Human)
HI   CVCL_0347 ! IMR-90
SX   Female
AG   16FW
CA   Finite cell line
DT   Created: 26-09-16; Last updated: 10-04-25; Version: 14
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RX   PubMed=9765202; DOI=10.1101/gad.12.19.2997; PMCID=PMC317194;
RA   Zhu J.-Y., Woods D., McMahon M., Bishop J.M.;
RT   "Senescence of human fibroblasts induced by oncogenic Raf.";
RL   Genes Dev. 12:2997-3007(1998).
//