ID   HaCaT HSP70B'-GFP reporter
AC   CVCL_FA29
DR   Wikidata; Q54872311
RX   PubMed=24967604;
CC   Population: Caucasian.
CC   Characteristics: Stably expresses GFP under the control of the stress-inducible HSPA6 (HSP70B') promoter (PubMed=24967604).
CC   Characteristics: Used as a sensor cell line to quantify skin response upon exposure to chemical substances that are known to cause irritation or allergies through the generation of reactive oxygen species (PubMed=24967604).
CC   Sequence variation: Mutation; HGNC; HGNC:11998; TP53; Simple; p.His179Tyr (c.535C>T); ClinVar=VCV000127815; Zygosity=Heterozygous (from parent cell line).
CC   Sequence variation: Mutation; HGNC; HGNC:11998; TP53; Simple; p.Arg282Trp (c.843_844CC>TT); Zygosity=Heterozygous (from parent cell line).
CC   Genetic integration: Method=Transfection; Gene=FPbase; R9NL8; eGFP (Note=Enhanced GFP).
CC   Derived from site: In situ; Back, skin, epidermis; UBERON=UBERON_0001068+UBERON_0001003.
CC   Cell type: Keratinocyte; CL=CL_0000312.
OX   NCBI_TaxID=9606; ! Homo sapiens (Human)
HI   CVCL_0038 ! HaCaT
SX   Male
AG   62Y
CA   Spontaneously immortalized cell line
DT   Created: 26-09-16; Last updated: 10-04-25; Version: 16
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RX   PubMed=24967604; DOI=10.3390/s140711293; PMCID=PMC4168474;
RA   Hofmann U., Priem M., Bartzsch C., Winckler T., Feller K.-H.;
RT   "A sensitive sensor cell line for the detection of oxidative stress
RT   responses in cultured human keratinocytes.";
RL   Sensors (Basel) 14:11293-11307(2014).
//