ID   HeLa S3 ATG13 KO+HaloTag-LC3
AC   CVCL_F1YZ
RX   DOI=10.1101/2025.11.07.687251;
CC   Population: African American.
CC   Characteristics: Stably expresses HaloTag-LC3 to measure autophagic flux.
CC   Knockout cell: Method=CRISPR/Cas9; HGNC; HGNC:29091; ATG13.
CC   Genetic integration: Method=Transduction; Gene=RGD; 621315; Map1lc3b.
CC   Genetic integration: Method=Transduction; Gene=UniProtKB; P00557; Escherichia coli hygromycin-B 4-O-kinase (hph) (HygR).
CC   Genetic integration: Method=Transduction; Gene=UniProtKB; P0A3G2; Rhodococcus rhodochrous dhaA (Note=Mutated = HaloTag).
CC   Transformant: NCBI_TaxID; 333761; Human papillomavirus type 18 (HPV18).
CC   Derived from site: In situ; Uterus, cervix; UBERON=UBERON_0000002.
DI   NCIt; C27677; Human papillomavirus-related endocervical adenocarcinoma
OX   NCBI_TaxID=9606; ! Homo sapiens (Human)
HI   CVCL_F1YY ! HeLa S3 ATG13 KO
SX   Female
AG   30Y6M
CA   Cancer cell line
DT   Created: 27-11-25; Last updated: 27-11-25; Version: 1
CH   CVCL_F1Z1 ! HeLa S3 ATG13 KO+Halo-LC3+HA-ATG13-HF:DD
CH   CVCL_F1Z0 ! HeLa S3 ATG13 KO+Halo-LC3+HA-ATG13-WT
//
RX   DOI=10.1101/2025.11.07.687251;
RA   Duan, Yong-Jia
RA   Lu, Ye
RA   Paul, Sanjoy
RA   Betz, Johannes
RA   Wilhelm, Lea P.
RA   Cook, Annan Samuel Isaac
RA   Ren, Xue-Feng
RA   Adriaenssens, Elias
RA   Martens, Sascha
RA   Ganley, Ian G.
RA   Hummer, Gerhard
RA   Hurley, James Herschel
RT   "Reconstitution of multistep recruitment of ULK1 to membranes in
RT   autophagy.";
RL   bioRxiv 2025:11.07.687251-11.07.687251(2025).
//