ID   TJPH-1
AC   CVCL_F0KZ
RX   PubMed=20355246;
CC   Characteristics: Transduced with the retroviral vector SSR#69 (MoMLV/LTR-LoxP-HygroR-HSV/TK-IRES-SV40T-LoxP-Neo-MoMLV/LTR) expressing HygroR, HSV-1 TK and SV40 T-antigen flanked by two loxP sites (PubMed=20355246).
CC   Characteristics: Because it has been transduced by a construct containing LoxP sites, transfection with the Cre recombinase eliminates the SV40 T gene thus reverting the cells to a non-immortal phenotype (PubMed=20355246).
CC   Genetic integration: Method=Transduction; Gene=UniProtKB; P00557; Escherichia coli hygromycin-B 4-O-kinase (hph) (HygR).
CC   Transformant: NCBI_TaxID; 1891767; Simian virus 40 (SV40).
CC   Derived from site: In situ; Liver; UBERON=UBERON_0002107.
CC   Cell type: Hepatocyte; CL=CL_0000182.
CC   Breed/subspecies: Miniature pig.
OX   NCBI_TaxID=9823; ! Sus scrofa (Pig)
SX   Male
AG   Age unspecified
CA   Transformed cell line
DT   Created: 14-08-25; Last updated: 14-08-25; Version: 1
//
RX   PubMed=20355246; DOI=10.3748/wjg.v16.i13.1660; PMCID=PMC2848376;
RA   Meng, Fan-Ying
RA   Chen, Zhi-Shui
RA   Han, Meng
RA   Hu, Xin-Peng
RA   He, Xing-Xing
RA   Liu, Yong
RA   He, Wen-Tao
RA   Huang, Wei
RA   Guo, Hui
RA   Zhou, Ping
RT   "Porcine hepatocyte isolation and reversible immortalization mediated
RT   by retroviral transfer and site-specific recombination.";
RL   World J. Gastroenterol. 16:1660-1664(2010).
//