ID   HT29-MTX resistant (1 microMolar)
AC   CVCL_F0KM
SY   HT29-MTX resistant (10-6M); HT29-MTX
DR   ECACC; 15121705
RX   PubMed=2205381;
RX   PubMed=8308060;
CC   Population: Caucasian.
CC   Characteristics: Resistant to 1 muM of methotrexate (PubMed=2205381).
CC   Selected for resistance to: ChEBI; CHEBI_44185; Methotrexate (MTX).
CC   Sequence variation: Mutation; HGNC; HGNC:583; APC; Simple; p.Glu853Ter (c.2557G>T); ClinVar=VCV001071632; Zygosity=Heterozygous (from parent cell line).
CC   Sequence variation: Mutation; HGNC; HGNC:583; APC; Simple; p.Thr1556Asnfs*3 (c.4666dupA) (c.4666_4667insA); ClinVar=VCV000428112; Zygosity=Heterozygous (from parent cell line).
CC   Sequence variation: Mutation; HGNC; HGNC:1097; BRAF; Simple; p.Val600Glu (c.1799T>A); ClinVar=VCV000013961; Zygosity=Heterozygous (from parent cell line).
CC   Sequence variation: Mutation; HGNC; HGNC:8975; PIK3CA; Simple; p.Pro449Thr (c.1345C>A); ClinVar=VCV001333250; Zygosity=Heterozygous (from parent cell line).
CC   Sequence variation: Mutation; HGNC; HGNC:6770; SMAD4; Simple; p.Gln311Ter (c.931C>T); ClinVar=VCV000230663; Zygosity=Homozygous (from parent cell line).
CC   Sequence variation: Mutation; HGNC; HGNC:11998; TP53; Simple; p.Arg273His (c.818G>A); ClinVar=VCV000012366; Zygosity=Homozygous (from parent cell line).
CC   Derived from site: In situ; Colon; UBERON=UBERON_0001155.
ST   Source(s): ECACC=15121705
ST   Amelogenin: X
ST   CSF1PO: 11,12
ST   D13S317: 11
ST   D16S539: 11,12
ST   D18S51: 13,20.2
ST   D21S11: 29
ST   D3S1358: 15,17
ST   D5S818: 11,12
ST   D7S820: 10
ST   D8S1179: 10
ST   FGA: 20,22
ST   Penta D: 11
ST   Penta E: 14,16
ST   TH01: 6,9
ST   TPOX: 8,9
ST   vWA: 17
DI   NCIt; C4349; Colon adenocarcinoma
OX   NCBI_TaxID=9606; ! Homo sapiens (Human)
HI   CVCL_0320 ! HT-29
SX   Female
AG   44Y
CA   Cancer cell line
DT   Created: 14-08-25; Last updated: 14-08-25; Version: 1
//
RX   PubMed=2205381;
RA   Lesuffleur, Thecla
RA   Barbat, Alain
RA   Dussaulx, Elisabeth
RA   Zweibaum, Alain
RT   "Growth adaptation to methotrexate of HT-29 human colon carcinoma
RT   cells is associated with their ability to differentiate into columnar
RT   absorptive and mucus-secreting cells.";
RL   Cancer Res. 50:6334-6343(1990).
//
RX   PubMed=8308060; DOI=10.1242/jcs.106.3.771;
RA   Lesuffleur, Thecla
RA   Porchet, Nicole
RA   Aubert, Jean-Pierre
RA   Swallow, Dallas M.
RA   Gum, James R. Jr.
RA   Kim, Young S.
RA   Real, Francisco X.
RA   Zweibaum, Alain
RT   "Differential expression of the human mucin genes MUC1 to MUC5 in
RT   relation to growth and differentiation of different mucus-secreting
RT   HT-29 cell subpopulations.";
RL   J. Cell Sci. 106:771-783(1993).
//