ID   WIZ03e-H9CAGhM3Dq
AC   CVCL_E9C5
DR   WiCell; wiz03e-h9caghm3dq
RX   PubMed=27133795;
CC   Registration: Swiss research registry; BAG-hES-IMP-0105.
CC   Characteristics: Expresses hM3Dq, a modified form of human CHRM3 that can no longer bind the native ligand, acetylcholine, but is activated by the inert clozapine metabolite clozapine-N-oxide (CNO), engaging the Gq signaling pathway (PubMed=27133795).
CC   Characteristics: Using CRISPR/Cas9 a T2A-PuroR-CAG-hM3Dq-mCherry construct was introduced in the AAVS1 safe harbor locus (PubMed=27133795).
CC   Genetic integration: Method=CRISPR/Cas9; Gene=FPbase; ZERB6; mCherry (Note=Red fluorescent protein derived from drFP583 (dsRed)).
CC   Genetic integration: Method=CRISPR/Cas9; Gene=HGNC; HGNC:1952; CHRM3 (Note=With p.Tyr149Cys and p.Ala239Gly = hM3Dq).
CC   Genetic integration: Method=CRISPR/Cas9; Gene=UniProtKB; P13249; Streptomyces alboniger pac (PuroR).
CC   Derived from site: In situ; Blastocyst; UBERON=UBERON_0000358.
CC   Cell type: Embryonic stem cell; CL=CL_0002322.
OX   NCBI_TaxID=9606; ! Homo sapiens (Human)
HI   CVCL_9773 ! WA09
SX   Female
AG   Blastocyst stage
CA   Embryonic stem cell
DT   Created: 14-08-25; Last updated: 14-08-25; Version: 1
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RX   PubMed=27133795; DOI=10.1016/j.stem.2016.03.014; PMCID=PMC4892985;
RA   Chen, Yue-Jun
RA   Xiong, Man
RA   Dong, Yi
RA   Haberman, Alexander
RA   Cao, Jing-Yuan
RA   Liu, Hui-Sheng
RA   Zhou, Wen-Hao
RA   Zhang, Su-Chun
RT   "Chemical control of grafted human PSC-derived neurons in a mouse
RT   model of Parkinson's disease.";
RL   Cell Stem Cell 18:817-826(2016).
//