ID   ICGi022-A-9
AC   CVCL_E9BB
SY   K7-4Lf MT21
DR   BioSamples; SAMEA117402930
DR   hPSCreg; ICGi022-A-9
RX   PubMed=40149527;
CC   From: The Federal Research Center Institute of Cytology and Genetics; Novosibirsk; Russia.
CC   Population: Caucasian; Siberian.
CC   Characteristics: Upon doxycycline induction expresses a MitoTimer biosensor. MitoTimer is a DsRed-Timer protein fused to the transit peptide of COX18 so as to target it to the mitochondrial matrix (PubMed=40149527; DOI=10.3390/biomedicines13030550; PMCID=PMC11940372).
CC   Characteristics: Using CRISPR/Cas9 a Neo-M2rtTA construct and a Puro-TRE-mCMV-MitoTimer were introduced at the AAVS1 safe harbor locus (PubMed=40149527).
CC   Genetic integration: Method=CRISPR/Cas9; Gene=FPbase; 55DQE; DsRed-Timer.
CC   Genetic integration: Method=CRISPR/Cas9; Gene=UniProtKB; P04483; Escherichia coli Tn10 tetR.
CC   Genetic integration: Method=CRISPR/Cas9; Gene=UniProtKB; P06492; Human herpesvirus 1 (HHV-1) VP16 (UL28) (Note=With p.Met1_Arg360del).
CC   Genetic integration: Method=CRISPR/Cas9; Gene=UniProtKB; P00552; Klebsiella pneumoniae transposon Tn5 neo.
CC   Genetic integration: Method=CRISPR/Cas9; Gene=UniProtKB; P13249; Streptomyces alboniger pac (PuroR).
CC   Derived from site: In situ; Peripheral blood; UBERON=UBERON_0000178.
OX   NCBI_TaxID=9606; ! Homo sapiens (Human)
HI   CVCL_ZE02 ! ICGi022-A
SX   Female
AG   42Y
CA   Induced pluripotent stem cell
DT   Created: 14-08-25; Last updated: 14-08-25; Version: 1
//
RX   PubMed=40149527; DOI=10.3390/biomedicines13030550; PMCID=PMC11940372;
RA   Nadtochy, Julia A.
RA   Medvedev, Sergey P.
RA   Grigor'eva, Elena V.
RA   Pavlova, Sofia V.
RA   Minina, Julia M.
RA   Chechushkov, Anton V.
RA   Malakhova, Anastasia A.
RA   Kovalenko, Liudmila V.
RA   Zakian, Suren Minasovich
RT   "Transgenic iPSC lines with genetically encoded MitoTimer to study
RT   mitochondrial biogenesis in dopaminergic neurons with tauopathy.";
RL   Biomedicines 13:550.1-550.18(2025).
//