ID   HS1_L1HS-CRISPRi_g1
AC   CVCL_E8I4
DR   GEO; GSM8667518
DR   GEO; GSM8667519
DR   GEO; GSM8667520
DR   PRIDE; PXD059661
RX   DOI=10.1101/2025.01.17.633315;
CC   Characteristics: Transduced with the FUGW U6 gL1HSg1dCas9-KRAB-T2a-GFP vector that contains dCas9 fused to a KRAB repressive domain, a LINE-1 targeting gRNA (gL1HSg1) and eGFP (DOI=10.1101/2025.01.17.633315).
CC   Genetic integration: Method=Transduction; Gene=FPbase; R9NL8; eGFP (Note=Enhanced GFP).
CC   Genetic integration: Method=Transduction; Gene=UniProtKB; Q99ZW2; Streptomyces pyogenes Cas9 (Note=With p.Asp10Ala and p.His840Ala and KRAB domain = dCas9-KRAB).
CC   Omics: Proteomics.
CC   Omics: Transcriptomics; RNAseq.
CC   Derived from site: In situ; Umbilical cord blood; UBERON=UBERON_0012168.
OX   NCBI_TaxID=9606; ! Homo sapiens (Human)
HI   CVCL_DQ11 ! 606A1
SX   Female
AG   <1D
CA   Induced pluripotent stem cell
DT   Created: 14-08-25; Last updated: 14-08-25; Version: 1
//
RX   DOI=10.1101/2025.01.17.633315;
RA   Adami, Anita
RA   Garza, Raquel
RA   Gerdes, Patricia
RA   Johansson, Pia Annette
RA   Dorazehi, Fereshteh
RA   Koutounidou, Symela
RA   Castilla-Vallmanya, Laura
RA   Atacho, Diahann Alexandra Maria
RA   Sharma, Yogita
RA   Johansson, Jenny G.
RA   Tam, Oliver H.
RA   Kirkeby, Agnete
RA   Barker, Roger Alistair
RA   Gale-Hammell, Molly C.
RA   Douse, Christopher H.
RA   Jakobsson, Johan
RT   "LINE-1 retrotransposons regulate the exit of human pluripotency and
RT   early brain development.";
RL   bioRxiv 2025:01.17.633315-01.17.633315(2025).
//