ID   S2R+-MT-sfGFP-TurboID-ER
AC   CVCL_E8G0
DR   DGRC; 351
DR   FlyBase_Cell_line; FBtc0000351
CC   Group: Insect cell line.
CC   Characteristics: Transfected with a MT-sfGFP-TurboID-ER construct containing a fusion of sfGFP with TurboID-ER, a modified biotin ligase targeted to the ER and used for proximity labeling to identify proteins interacting with a protein of interest. The expression of the fusion protein is driven by a copper-inducible metallothionein promoter (DGRC=351).
CC   Genetic integration: Method=Transfection; Gene=FPbase; B4SOW; Superfolder GFP (Note=sfGFP, superfolding variant of GFP).
CC   Genetic integration: Method=Transfection; Gene=UniProtKB; P06709; Escherichia coli birA (Note=With p.Gln65Pro, p.Ile87Val, p.Arg118Ser, p.Glu140Lys, p.Gln141Arg, p.Ala146del, p.Ser150Gly, p.Leu151Pro, p.Val160Ala, p.Thr192Ala, p.Lys194Ile, p.Met209Val, p.Met241Thr, p.Ser263Pro and p.Ile305Val and with a C-terminal KDEL tag = TurboID-ER).
CC   Genetic integration: Method=Transfection; Gene=UniProtKB; P00557; Escherichia coli hygromycin-B 4-O-kinase (hph) (HygR).
CC   Derived from site: In situ; Whole embryo; UBERON=UBERON_0000922.
CC   Breed/subspecies: Oregon-R; FlyBase_Strain=FBsn0000276.
OX   NCBI_TaxID=7227; ! Drosophila melanogaster (Fruit fly)
HI   CVCL_Z831 ! S2R+
SX   Male
AG   Late embryonic stage
CA   Spontaneously immortalized cell line
DT   Created: 14-08-25; Last updated: 14-08-25; Version: 1
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