ID   Applied StemCell DR4-MEF mitomycin-treated
AC   CVCL_E8D3
WW   Provider; ASC; ASF-1023 to ASF-1026; https://appliedstemcell.com/mef-feeder-cells/
CC   Characteristics: Expresses transgenes that make this cell line resistant to three different drugs: geneticin (G418), puromycin and hygromycin. KO of Hprt1 makes the cell line resistant to 6-tioguanine (PubMed=9278500).
CC   Characteristics: Used as feeder cells to support the growth of stem cells in the undifferentiated state.
CC   Characteristics: Growth-arrested by mitomycin C.
CC   Knockout cell: Method=KO mouse; MGI; MGI:96217; Hprt1.
CC   Genetic integration: Method=Transgenic mouse; Gene=UniProtKB; P00557; Escherichia coli hygromycin-B 4-O-kinase (hph) (HygR).
CC   Genetic integration: Method=Transgenic mouse; Gene=UniProtKB; P13249; S.alboniger pac (PuroR).
CC   Genetic integration: Method=Transgenic mouse; Gene=UniProtKB; P00552; Transposon Tn5 neo.
CC   Derived from site: In situ; Embryo; UBERON=UBERON_0000922.
CC   Cell type: Fibroblast; CL=CL_0000057.
CC   Breed/subspecies: DR4.
OX   NCBI_TaxID=10090; ! Mus musculus (Mouse)
SX   Sex unspecified
AG   13-14FD
CA   Finite cell line
DT   Created: 10-04-25; Last updated: 10-04-25; Version: 1
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