ID   iLite Type I IFN Assay Ready Cells
AC   CVCL_E7RW
RX   Patent=US8759091;
WW   Provider; Svar Life Science; BM3049; https://www.svarlifescience.com/products/bm3049
CC   Population: Caucasian.
CC   Characteristics: Engineered to express firefly Luciferase under the control of an IFN alpha/beta responsive promoter. When IFN alpha or IFN beta binds to the IFN a/b receptor on the cell surface, the IFN a/b regulated firefly luciferase reporter gene construct is activated, resulting in a luminescent signal (Svar Life Science=BM3049).
CC   Sequence variation: Gene fusion; HGNC; HGNC:16063; MLLT10 + HGNC; HGNC:15514; PICALM; Name(s)=PICALM-MLLT10, CALM-AF10 (from parent cell line).
CC   Sequence variation: Mutation; HGNC; HGNC:9588; PTEN; Simple; p.Gly129fs*51 (c.387_388insCGCC); Zygosity=Hemizygous (from parent cell line).
CC   Sequence variation: Mutation; HGNC; HGNC:9644; PTPN11; Simple; p.Gly60Arg (c.178G>C); ClinVar=VCV000372590; Zygosity=Heterozygous (from parent cell line).
CC   Sequence variation: Mutation; HGNC; HGNC:11998; TP53; Simple; c.559+1G>A (p.Val173Trpfs*59); ClinVar=VCV000428908; Zygosity=Homozygous; Note=Splice donor mutation (from parent cell line).
CC   Sequence variation: Mutation; HGNC; HGNC:12796; WT1; Simple; p.Arg301Ter (c.901C>T) (p.Arg352Ter, c.1054C>T) (p.Arg374Ter, c.1120C>T); ClinVar=VCV000449416; Zygosity=Heterozygous (from parent cell line).
CC   Genetic integration: Method=Transfection; Gene=UniProtKB; P08659; Firefly luciferase.
CC   Genetic integration: Method=Transfection; Gene=UniProtKB; P00552; Transposon Tn5 neo.
CC   Derived from site: In situ; Pleural effusion; UBERON=UBERON_0000175.
DI   NCIt; C8263; Adult acute monocytic leukemia
DI   ORDO; Orphanet_514; Acute monoblastic/monocytic leukemia
OX   NCBI_TaxID=9606; ! Homo sapiens (Human)
HI   CVCL_0007 ! U-937
SX   Male
AG   37Y
CA   Cancer cell line
DT   Created: 10-04-25; Last updated: 10-04-25; Version: 1
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RX   Patent=US8759091;
RA   Lallemand C., Tovey M.G.;
RT   "Gene reporter assay, kit and cells with improved sensitivity and/or
RT   specificity for determining the level of an extracellular signal.";
RL   Patent number US8759091, 24-Jun-2014.
//