ID   iLite IL-23 Assay Ready Cells
AC   CVCL_E7RR
SY   DT4023L
RX   CelloPub=CLPUB00797;
RX   PubMed=32484379;
WW   Provider; Svar Life Science; BM4023; https://www.svarlifescience.com/products/bm4023
CC   Group: Bird cell line.
CC   Characteristics: Genetically engineered and optimized to be responsive to IL23, resulting in the specific expression of firefly luciferase. Normalization of cell counts and serum matrix effects are obtained by a second reporter gene, a Renilla-type luciferase reporter gene construct, under control of a constitutive promoter (Svar Life Science=BM4023).
CC   Characteristics: Transfected with the IL23R and IL12RB1 receptor chains both under the control of a CMV reporter, firefly luciferase under the control of STAT5 responsive element and renilla luciferase under the control of a CMV promoter (CelloPub=CLPUB00797).
CC   Genetic integration: Method=Transfection; Gene=HGNC; HGNC:5971; IL12RB1.
CC   Genetic integration: Method=Transfection; Gene=HGNC; HGNC:19100; IL23R.
CC   Genetic integration: Method=Transfection; Gene=UniProtKB; P08659; Firefly luciferase.
CC   Genetic integration: Method=Transfection; Gene=UniProtKB; P27652; Renilla-type luciferase.
CC   Transformant: NCBI_TaxID; 11864; Avian leukosis virus (ALV).
CC   Cell type: B-cell; CL=CL_0000236.
CC   Breed/subspecies: Hy-Line SC.
DI   NCIt; C134556; Chicken bursal lymphoma
OX   NCBI_TaxID=9031; ! Gallus gallus (Chicken)
HI   CVCL_0249 ! DT40
SX   Female
AG   1D
CA   Cancer cell line
DT   Created: 10-04-25; Last updated: 10-04-25; Version: 1
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RX   CelloPub=CLPUB00797;
RA   Lallemand C., Tovey M.G.;
RT   "iLite reporter gene assay for quantification of the activity of
RT   anti-IL-23 and anti-IL-23 neutralizing antibodies.";
RL   (In conference) 11th annual Essential Protein Engineering Summit (PEGS Boston); pp.E135-E135; Cambridge HealthTech Institute; Boston; USA (2015).
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RX   PubMed=32484379; DOI=10.1177/2472555220923362;
RA   Varghese T.M., Dudas P.L., Allen S.J., Schneeweis J.E., Finley M.F.A.;
RT   "Optimization of a high-throughput cell-based screening strategy to
RT   identify small-molecule inhibitors of IL-23 signaling.";
RL   SLAS Discov. 26:122-129(2021).
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