ID   hURO18H7 IQAD biosensor
AC   CVCL_E7CP
SY   hURO18H7 caspase-1 biosensor
RX   CelloPub=CLPUB00789;
CC   Characteristics: Can be used as a biosensor to measure caspase-1 induced cellular inflammatory responses to when infected bacterial urinary pathogens (CelloPub=CLPUB007899).
CC   Characteristics: Transduced with a firefly luciferase reporter circularly permuted in which the N- and C-terminal domains are physically separated by a flexible hinge region containing a caspase-1 cleaving site (IQADSGPIG) (CelloPub=CLPUB00789).
CC   Genetic integration: Method=Transduction; Gene=UniProtKB; P08659; Firefly luciferase (Note=Circ. permuted with an IQAD caspase-1 site).
CC   Genetic integration: Method=Transduction; Gene=UniProtKB; P03126; Human papillomavirus type 16 protein E6.
CC   Genetic integration: Method=Transduction; Gene=UniProtKB; P03129; Human papillomavirus type 16 protein E7.
CC   Genetic integration: Method=Transduction; Gene=UniProtKB; P13249; S.alboniger pac (PuroR).
CC   Genetic integration: Method=Transduction; Gene=UniProtKB; P00552; Transposon Tn5 neo.
CC   Transformant: NCBI_TaxID; 333760; Human papillomavirus type 16 (HPV16).
CC   Donor information: Established from a perimenopausal female patient undergoing urogynecological surgery for stress urinary incontinence (from parent cell line).
CC   Derived from site: In situ; Urinary bladder; UBERON=UBERON_0001255.
CC   Cell type: Urothelial cell; CL=CL_0000731.
OX   NCBI_TaxID=9606; ! Homo sapiens (Human)
HI   CVCL_E7CN ! hURO18H7
SX   Female
AG   Adult
CA   Transformed cell line
DT   Created: 10-04-25; Last updated: 10-04-25; Version: 1
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RX   CelloPub=CLPUB00789;
RA   Kesav N.R.;
RT   "Development of a urothelial cell line expressing a caspase-1
RT   biosensor to measure cellular inflammatory responses to potential
RT   uropathogens.",
RL   Thesis MSc (2024); Loyola University of Chicago; Chicago; USA.
//