ID   FIRE2FLIPCS clone 1
AC   CVCL_E6HT
SY   FIRE2FLPCS-520-8
RX   PubMed=39824193;
CC   Characteristics: Expresses a Fe-IRE (FIRE) biosensor that senses IRP activity and cellular iron status (PubMed=39824193).
CC   Characteristics: This cell line contains a FIRE2 biosensor (emiRFP670-sensor, mRuby3-control) and is transposed with two PiggyBac constructs FIRES-FLIP-PBCS-E670 and FIREC-PBCS-MR3 flanking FIRE sensor and control fluorescent protein, respectively. The FLIP-FIRE sensor is composed of a 740 bp sequence synthesized in backwards orientation and based on known mammalian IRP1/2 binding sites (PubMed=39824193).
CC   Genetic integration: Method=PiggyBac transposition; Gene=FPbase; YHJV5; emiRFP670 (Note=Constitutively fluorescent near IR fluoresent protein from R.palustris).
CC   Genetic integration: Method=PiggyBac transposition; Gene=FPbase; MZMFB; mRuby3 (Note=Bright red fluorescent protein derived from eqFP611).
CC   Derived from site: In situ; Blastocyst; UBERON=UBERON_0000358.
CC   Cell type: Embryonic stem cell; CL=CL_0002322.
CC   Breed/subspecies: C57BL/6J x 129S4/SvJae.
OX   NCBI_TaxID=10090; ! Mus musculus (Mouse)
HI   CVCL_C865 ! v6.5
SX   Male
AG   Blastocyst stage
CA   Embryonic stem cell
DT   Created: 10-04-25; Last updated: 10-04-25; Version: 1
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RX   PubMed=39824193; DOI=10.1016/j.crmeth.2024.100960;
RA   Sangokoya C.;
RT   "The FIRE biosensor illuminates iron regulatory protein activity and
RT   cellular iron homeostasis.";
RL   Cell Rep. Methods 5:100960.1-100960.8(2025).
//