ID   FIRE1CS clone 1
AC   CVCL_E6HQ
SY   FIRE1CS-178A3
RX   PubMed=39824193;
CC   Characteristics: Expresses a Fe-IRE (FIRE) biosensor that senses IRP activity and cellular iron status (PubMed=39824193).
CC   Characteristics: This cell line contains a FIRE1 biosensor (mClover3-sensor, mCherry-control) and is transposed with two PiggyBac constructs FIRES-PBCS-CLV3 and FIREC-PBCS-MC2 flanking FIRE sensor and control fluorescent protein, respectively. The FIRE sensor is composed of a 740 bp sequence synthesized based on known mammalian IRP1/2 binding sites (PubMed=39824193).
CC   Genetic integration: Method=PiggyBac transposition; Gene=FPbase; ZERB6; mCherry (Note=Red fluorescent protein derived from drFP583 (dsRed)).
CC   Genetic integration: Method=PiggyBac transposition; Gene=FPbase; RYFE4; mClover3 (Note=Basic green/yellow fluorescent protein derived from GFP).
CC   Derived from site: In situ; Blastocyst; UBERON=UBERON_0000358.
CC   Cell type: Embryonic stem cell; CL=CL_0002322.
CC   Breed/subspecies: C57BL/6J x 129S4/SvJae.
OX   NCBI_TaxID=10090; ! Mus musculus (Mouse)
HI   CVCL_C865 ! v6.5
SX   Male
AG   Blastocyst stage
CA   Embryonic stem cell
DT   Created: 10-04-25; Last updated: 10-04-25; Version: 1
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RX   PubMed=39824193; DOI=10.1016/j.crmeth.2024.100960;
RA   Sangokoya C.;
RT   "The FIRE biosensor illuminates iron regulatory protein activity and
RT   cellular iron homeostasis.";
RL   Cell Rep. Methods 5:100960.1-100960.8(2025).
//