ID   U2OS-CRISPR-SNAPf-NUP133 no.80
AC   CVCL_E6B0
SY   U2OS-CRISPR-SNAPf-Nup133 #80; U2OS CRISPR SNAPf-NUP133 #80
DR   CLS; 300666
RX   PubMed=34191541;
CC   Population: Caucasian.
CC   Characteristics: Using CRISPR/Cas9D10A NUP1333 has been endogenously tagged in both alleles at the N-terminus with a SNAP-f tag (PubMed=34191541).
CC   Sequence variation: Mutation; HGNC; HGNC:9277; PPM1D; Simple; p.Arg458Ter (c.1372C>T); ClinVar=VCV002445214; Zygosity=Heterozygous (from parent cell line).
CC   Genetic integration: Method=CRISPR/Cas9; Gene=HGNC; HGNC:7059; MGMT (Note=With p.Gly183_Asn207del and 20 mutations = SNAP-f-tag).
CC   Derived from site: In situ; Bone, tibia; UBERON=UBERON_0000979.
DI   NCIt; C9145; Osteosarcoma
DI   ORDO; Orphanet_668; Osteosarcoma
OX   NCBI_TaxID=9606; ! Homo sapiens (Human)
HI   CVCL_0042 ! U2OS
SX   Female
AG   15Y
CA   Cancer cell line
DT   Created: 10-04-25; Last updated: 10-04-25; Version: 1
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RX   PubMed=34191541; DOI=10.1091/mbc.E20-11-0728; PMCID=PMC8351745;
RA   Sabinina V.J., Hossain M.J., Heriche J.-K., Hoess P.,
RA   Nijmeijer-Winter B., Mosalaganti S., Kueblbeck M., Callegari A.,
RA   Szymborska A., Beck M., Ries J., Ellenberg J.;
RT   "Three-dimensional superresolution fluorescence microscopy maps the
RT   variable molecular architecture of the nuclear pore complex.";
RL   Mol. Biol. Cell 32:1523-1533(2021).
//