ID   Abcam U-87MG STXBP1 KO
AC   CVCL_E4AP
SY   Human STXBP1 knockout U-87 MG
DR   Abcam; ab322392
RX   DOI=10.12688/f1000research.160174.1;
CC   Problematic cell line: Misidentified. Parent cell line (U-87MG ATCC) is not the original glioblastoma cell line established in 1968 at the University of Uppsala.
CC   Population: Caucasian.
CC   Knockout cell: Method=CRISPR/Cas9; HGNC; HGNC:11444; STXBP1.
CC   Sequence variation: Mutation; HGNC; HGNC:7765; NF1; Simple; p.Phe1247Ilefs*18 (c.3739_3742delTTTG) (3737_3740delTGTT); ClinVar=VCV000420078; Zygosity=Heterozygous (from parent cell line).
CC   Sequence variation: Mutation; HGNC; HGNC:9588; PTEN; Simple; c.209+1G>T; ClinVar=VCV000229705; Zygosity=Homozygous; Note=Splice donor mutation (from parent cell line).
CC   Sequence variation: Mutation; HGNC; HGNC:11730; TERT; Simple; c.1-124C>T (c.228C>T) (C228T); Zygosity=Unspecified; Note=In promoter (from parent cell line).
DI   NCIt; C3058; Glioblastoma
DI   ORDO; Orphanet_360; Glioblastoma
OX   NCBI_TaxID=9606; ! Homo sapiens (Human)
HI   CVCL_0022 ! U-87MG ATCC
SX   Male
AG   Age unspecified
CA   Cancer cell line
DT   Created: 19-12-24; Last updated: 10-04-25; Version: 2
//
RX   DOI=10.12688/f1000research.160174.1;
RA   Ayoubi R., Alende C., Fotouhi M., Gonzalez Bolivar S., Laflamme C.;
RG   ABIF Consortium;
RG   NeuroSGC/YCharOS/EDDU Collaborative Group;
RT   "A guide to selecting high-performing antibodies for STXBP1 (UniProt ID:
RT   P61764) for use in western blot, immunoprecipitation, and
RT   immunofluorescence.";
RL   F1000Research 13:1557.1-1557.12(2024).
//