ID   EPC-EC-Vip-C11
AC   CVCL_E3AK
RX   PubMed=38951796;
CC   Group: Fish cell line.
CC   Problematic cell line: Misidentified. Grand-parent cell line (EPC) was originally deposited as a carp (Cyprinus carpio) cell line, is in fact a fathead minnow (Pimephales promelas) cell line.
CC   Characteristics: The parent cell line was transiently transfected with a mix of sgRNAs designed against stat2 and against mEGFP. mEGFP-deficient cells were isolated (PubMed=38951796).
CC   Knockout cell: Method=CRISPR/Cas9; UniProtKB; A0A096ZN79; Viperin (Vig1).
CC   Genetic integration: Method=Transfection; Gene=FPbase; R9NL8; eGFP (Note=Enhanced GFP).
CC   Genetic integration: Method=Transfection; Gene=UniProtKB; Q99ZW2; Streptomyces pyogenes Cas9 (Note=Nuclear version; nCas9n).
CC   Genetic integration: Method=Transfection; Gene=UniProtKB; P00557; Escherichia coli hygromycin-B 4-O-kinase (hph) (HygR).
CC   Genetic integration: Method=Transfection; Gene=UniProtKB; P00552; Klebsiella pneumoniae transposon Tn5 neo.
CC   Cell type: Epithelial cell; CL=CL_0000066.
OX   NCBI_TaxID=90988; ! Pimephales promelas (Fathead minnow)
HI   CVCL_E3AG ! EPC-EC
SX   Sex unspecified
AG   Age unspecified
CA   Spontaneously immortalized cell line
DT   Created: 10-09-24; Last updated: 14-08-25; Version: 3
//
RX   PubMed=38951796; DOI=10.1186/s12864-024-10566-x; PMCID=PMC11218377;
RA   Chaumont, Lise
RA   Jouneau, Luc
RA   Huetz, Francois
RA   van Muilekom, Doret R.
RA   Peruzzi, Mathilde
RA   Raffy, Claudine
RA   Le Hir, Jerome
RA   Minke, Jules Maarten H.M.
RA   Boudinot, Pierre
RA   Collet, Bertrand
RT   "Unexpected regulatory functions of cyprinid viperin on inflammation
RT   and metabolism.";
RL   BMC Genomics 25:650.1-650.28(2024).
//