ID   CHSE-EC
AC   CVCL_DG46
SY   EC
DR   Wikidata; Q54813318
RX   PubMed=27236514;
RX   PubMed=32576161;
CC   Group: Fish cell line.
CC   Genetic integration: Method=Transfection; Gene=FPbase; R9NL8; eGFP (Note=Enhanced GFP).
CC   Genetic integration: Method=Transfection; Gene=UniProtKB; Q99ZW2; Streptomyces pyogenes Cas9 (Note=Nuclear version; nCas9n).
CC   Genetic integration: Method=Transfection; Gene=UniProtKB; P00557; Escherichia coli hygromycin-B 4-O-kinase (hph) (HygR).
CC   Genetic integration: Method=Transfection; Gene=UniProtKB; P00552; Klebsiella pneumoniae transposon Tn5 neo.
CC   Derived from site: In situ; Whole embryo; UBERON=UBERON_0000922.
CC   Cell type: Epithelial cell; CL=CL_0000066.
OX   NCBI_TaxID=74940; ! Oncorhynchus tshawytscha (Chinook salmon)
HI   CVCL_DG45 ! CHSE-E
SX   Sex unspecified
AG   Embryo
CA   Spontaneously immortalized cell line
DT   Created: 13-07-16; Last updated: 14-08-25; Version: 13
CH   CVCL_E3AE ! CHSE-EC-PKR-C19
CH   CVCL_E3AF ! CHSE-EC-PKR-C28
CH   CVCL_E3AC ! CHSE-EC-PKR-C3
CH   CVCL_E3AD ! CHSE-EC-PKR-C5
CH   CVCL_YI85 ! CHSE-GS2
//
RX   PubMed=32576161; DOI=10.1186/s12896-020-00626-x; PMCID=PMC7310381;
RA   Gratacap, Remi L.
RA   Regan, Tim
RA   Dehler, Carola E.
RA   Martin, Samuel A.M.
RA   Boudinot, Pierre
RA   Collet, Bertrand
RA   Houston, Ross D.
RT   "Efficient CRISPR/Cas9 genome editing in a salmonid fish cell line
RT   using a lentivirus delivery system.";
RL   BMC Biotechnol. 20:35.1-35.9(2020).
//
RX   PubMed=27236514; DOI=10.1007/s10126-016-9708-6; PMCID=PMC5007268;
RA   Dehler, Carola E.
RA   Boudinot, Pierre
RA   Martin, Samuel A.M.
RA   Collet, Bertrand
RT   "Development of an efficient genome editing method by CRISPR/Cas9 in a
RT   fish cell line.";
RL   Mar. Biotechnol. 18:449-452(2016).
//