ID   CHSE-EC
AC   CVCL_DG46
SY   EC
DR   Wikidata; Q54813318
RX   PubMed=27236514;
RX   PubMed=32576161;
CC   Group: Fish cell line.
CC   Genetic integration: Method=Transfection; Gene=FPbase; R9NL8; eGFP (Note=Enhanced GFP).
CC   Genetic integration: Method=Transfection; Gene=UniProtKB; Q99ZW2; Streptomyces pyogenes Cas9 (Note=Nuclear version; nCas9n).
CC   Genetic integration: Method=Transfection; Gene=UniProtKB; P00557; Escherichia coli hygromycin-B 4-O-kinase (hph) (HygR).
CC   Genetic integration: Method=Transfection; Gene=UniProtKB; P00552; Transposon Tn5 neo.
CC   Derived from site: In situ; Whole embryo; UBERON=UBERON_0000922.
CC   Cell type: Epithelial cell; CL=CL_0000066.
OX   NCBI_TaxID=74940; ! Oncorhynchus tshawytscha (Chinook salmon)
HI   CVCL_DG45 ! CHSE-E
SX   Sex unspecified
AG   Embryo
CA   Spontaneously immortalized cell line
DT   Created: 13-07-16; Last updated: 10-04-25; Version: 12
//
RX   PubMed=27236514; DOI=10.1007/s10126-016-9708-6; PMCID=PMC5007268;
RA   Dehler C.E., Boudinot P., Martin S.A.M., Collet B.;
RT   "Development of an efficient genome editing method by CRISPR/Cas9 in a
RT   fish cell line.";
RL   Mar. Biotechnol. 18:449-452(2016).
//
RX   PubMed=32576161; DOI=10.1186/s12896-020-00626-x; PMCID=PMC7310381;
RA   Gratacap R.L., Regan T., Dehler C.E., Martin S.A.M., Boudinot P.,
RA   Collet B., Houston R.D.;
RT   "Efficient CRISPR/Cas9 genome editing in a salmonid fish cell line
RT   using a lentivirus delivery system.";
RL   BMC Biotechnol. 20:35.1-35.9(2020).
//